The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae

PDK1 (phosphoinositide-dependent protein kinase 1) phosphorylates and activates PKA (cAMP-dependent protein kinase) in vitro. Docking of the HM (hydrophobic motif) in the C-terminal tail of the PKA catalytic subunits on to the PIF (PDK1-interacting fragment) pocket of PDK1 is a critical step in this...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Haesendonckx, S., Tudisca, V., Voordeckers, K., Moreno, S., Thevelein, J.M., Portela, P.
Formato: JOUR
Materias:
Bcy1
cAMP-dependent protein kinase (PKA)
Phosphoinositide-dependent protein kinase 1 (PDK1)
PKB (protein kinase B)-activating kinase homologue (Pkh)
Saccharomyces cerevisiae
Tpk
cyclic AMP dependent protein kinase
fungal enzyme
glutathione transferase
hybrid protein
phosphoinositide dependent protein kinase 1
protein kinase B activating kinase homologue
unclassified drug
article
catalysis
culture medium
enzyme activation
enzyme active site
enzyme assay
enzyme phosphorylation
enzyme purification
Escherichia coli
fungus culture
fungus growth
in vitro study
in vivo study
nonhuman
plasmid
polyacrylamide gel electrophoresis
priority journal
protein protein interaction
Western blotting
yeast
Amino Acid Motifs
Amino Acid Sequence
Catalytic Domain
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits
Cyclic AMP-Dependent Protein Kinase Type I
Cyclic AMP-Dependent Protein Kinases
Enzyme Activation
Isoenzymes
Molecular Sequence Data
Mutagenesis
Phosphorylation
Protein-Serine-Threonine Kinases
Saccharomyces cerevisiae Proteins
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02646021_v448_n3_p307_Haesendonckx
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

Ejemplares similares

Ejemplares similares