Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel

(125I)-ovine prolactin (oPRL) binding was found in several brain areas of the toad, Bufo arenarum Hensel. The olfactory bulb, cerebral hemispheres, and both dorsal and ventral mesencephalic regions showed saturable, high affinity, (125I)-oPRL binding, ranging between 5.6 to 29.9 fmol/mg protein, whi...

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Detalles Bibliográficos
Autores principales: Lüthy, I.A., Segura, E.T., Lüthy, V.I., Charreau, E.H., Calandra, R.S.
Formato: JOUR
Materias:
hormone receptor
prolactin i 125
radioisotope
animal cell
central nervous system
drug receptor binding
kidney
nonhuman
pharmacokinetics
priority journal
Animals
Binding, Competitive
Brain
Bufo arenarum
Kidney
Kinetics
Male
Prolactin
Receptors, Cell Surface
Receptors, Prolactin
Sheep
Tissue Distribution
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01741578_v155_n5_p611_Luthy
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:(125I)-ovine prolactin (oPRL) binding was found in several brain areas of the toad, Bufo arenarum Hensel. The olfactory bulb, cerebral hemispheres, and both dorsal and ventral mesencephalic regions showed saturable, high affinity, (125I)-oPRL binding, ranging between 5.6 to 29.9 fmol/mg protein, while the association constant (Ka) by Scatchard analysis was between 4.0 to 8.7×109 M-1. This binding was compared with the Scatchard plot of the kidney, which has been already described by other groups, and gave 41.7 fmol/mg protein and Ka 2.5×109 M-1. Liver showed no binding and in the cerebral hemispheres (125I)-oPRL was not displaced by non-lactogenic hormones, indicating that binding was hormone and tissue specific. © 1985 Springer-Verlag.

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