Accumulation of hEGF and hEGF-fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light

Chloroplast transformation has many potential advantages for the production of recombinant proteins in plants. However, it has been reported that heterologous protein accumulation in chloroplasts could be hindered by post-transcriptional mechanisms not yet characterized. Here, we describe the develo...

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Detalles Bibliográficos
Autores principales: Wirth, S., Segretin, M.E., Mentaberry, A., Bravo-Almonacid, F.
Formato: JOUR
Materias:
Chloroplast transformation
Fusion protein
hEGF
Molecular farming
Transplastomic tobacco
Plants (botany)
Tobacco
Electron transport inhibitors
Human epidermal growth factor (hEGF)
Proteins
amino acid
bacterial enzyme
beta glucuronidase
gamma urogastrone
hybrid protein
protein
urea derivative
viologen
article
chloroplast
controlled study
electron transport
genetic transcription
incubation time
light dark cycle
nonhuman
nucleotide sequence
plant
plant leaf
priority journal
protein expression
Blotting, Northern
Blotting, Southern
Blotting, Western
Chloroplasts
Epidermal Growth Factor
Gene Expression Regulation, Plant
Genetic Vectors
Glucuronidase
Humans
Light
Plants, Genetically Modified
Recombinant Fusion Proteins
Chlorophylls
Plants
Plastids
Bacteria (microorganisms)
Nicotiana obtusifolia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01681656_v125_n2_p159_Wirth
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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