New methods for spectrofluorometer monochromator wavelength calibration

A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristics absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of f...

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Detalles Bibliográficos
Autores principales:	Paladini, A.A., Erijman, L.
Formato:	JOUR
Materias:	Calibration Didymium filter Holmium filter Instrumentation Monochrmator Wavelength article calibration instrumentation spectrofluorometry standard Spectrometry, Fluorescence Weights and Measures
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0165022X_v17_n1_p61_Paladini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0165022X_v17_n1_p61_Paladini
record_format	dspace
spelling	todo:paper_0165022X_v17_n1_p61_Paladini2023-10-03T15:02:32Z New methods for spectrofluorometer monochromator wavelength calibration Paladini, A.A. Erijman, L. Calibration Didymium filter Holmium filter Instrumentation Monochrmator Wavelength article calibration instrumentation spectrofluorometry standard Calibration Spectrometry, Fluorescence Weights and Measures A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristics absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of fluorophores like indole, diphenyl hexatriene, xylene or rhodamine 6G. Those filters or a well characterized absorber with sharp bands like benzene vapor can be used for the same purpose. The wavelength calibration accuracy obtained with this method is better than 0.1 nm, and requires no modification in the geometry of the spectrofluorometer sample compartment. © 1988. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0165022X_v17_n1_p61_Paladini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Calibration Didymium filter Holmium filter Instrumentation Monochrmator Wavelength article calibration instrumentation spectrofluorometry standard Calibration Spectrometry, Fluorescence Weights and Measures
spellingShingle	Calibration Didymium filter Holmium filter Instrumentation Monochrmator Wavelength article calibration instrumentation spectrofluorometry standard Calibration Spectrometry, Fluorescence Weights and Measures Paladini, A.A. Erijman, L. New methods for spectrofluorometer monochromator wavelength calibration
topic_facet	Calibration Didymium filter Holmium filter Instrumentation Monochrmator Wavelength article calibration instrumentation spectrofluorometry standard Calibration Spectrometry, Fluorescence Weights and Measures
description	A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristics absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of fluorophores like indole, diphenyl hexatriene, xylene or rhodamine 6G. Those filters or a well characterized absorber with sharp bands like benzene vapor can be used for the same purpose. The wavelength calibration accuracy obtained with this method is better than 0.1 nm, and requires no modification in the geometry of the spectrofluorometer sample compartment. © 1988.
format	JOUR
author	Paladini, A.A. Erijman, L.
author_facet	Paladini, A.A. Erijman, L.
author_sort	Paladini, A.A.
title	New methods for spectrofluorometer monochromator wavelength calibration
title_short	New methods for spectrofluorometer monochromator wavelength calibration
title_full	New methods for spectrofluorometer monochromator wavelength calibration
title_fullStr	New methods for spectrofluorometer monochromator wavelength calibration
title_full_unstemmed	New methods for spectrofluorometer monochromator wavelength calibration
title_sort	new methods for spectrofluorometer monochromator wavelength calibration
url	http://hdl.handle.net/20.500.12110/paper_0165022X_v17_n1_p61_Paladini
work_keys_str_mv	AT paladiniaa newmethodsforspectrofluorometermonochromatorwavelengthcalibration AT erijmanl newmethodsforspectrofluorometermonochromatorwavelengthcalibration
_version_	1807322221910163456

New methods for spectrofluorometer monochromator wavelength calibration

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