Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus
The use of biomarkers as a tool to assess responses of organisms exposed to pollutants in toxicity bioassays, as well as in aquatic environmental risk assessment protocols, requires the understanding of the natural fluctuation of the particular biomarker. The aim of this study was to characterize th...
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todo:paper_01476513_v111_n_p236_MenendezHelman2023-10-03T15:00:36Z Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus Menéndez-Helman, R.J. Ferreyroa, G.V. dos Santos Afonso, M. Salibián, A. Acetylcholinesterase Biomarkers Circannual rhythms Cnesterodon decemmaculatus Seasonal variations acetylcholinesterase acetylcholinesterase biological marker fresh water water pollutant bioassay biomarker environmental risk freshwater environment pollution exposure protein seasonal variation teleost toxicity animal experiment animal tissue Article biological monitoring biological trait circannual rhythm Cnesterodon decemmaculatus controlled study ecotoxicology environmental impact assessment environmental stress enzyme activity enzyme mechanism fish length nonhuman physical chemistry physical parameters risk assessment seasonal variation summer teleost toxicity testing winter animal body size Cyprinodontiformes environmental monitoring metabolism photoperiodicity procedures season temperature toxicity water pollutant Cnesterodon decemmaculatus Acetylcholinesterase Animals Biological Markers Body Size Cyprinodontiformes Environmental Monitoring Fresh Water Photoperiod Seasons Temperature Water Pollutants, Chemical The use of biomarkers as a tool to assess responses of organisms exposed to pollutants in toxicity bioassays, as well as in aquatic environmental risk assessment protocols, requires the understanding of the natural fluctuation of the particular biomarker. The aim of this study was to characterize the intrinsic variations of acetylcholinesterase (AChE) activity in tissues of a native freshwater teleost fish to be used as biomarker in toxicity tests, taking into account both seasonal influence and fish size.Specific AChE activity was measured by the method of Ellman et al. (1961) in homogenates of fish anterior section finding a seasonal variability. The highest activity was observed in summer, decreasing significantly below 40% in winter. The annual AChE activity cycle in the anterior section was fitted to a sinusoidal function with a period of 11.2 months. Moreover, an inverse relationship between enzymatic activity and the animal size was established. The results showed that both the fish length and seasonal variability affect AChE activity.AChE activity in fish posterior section showed a similar trend to that in the anterior section, while seasonal variations of the activity in midsection were observed but differences were not statistically significant.In addition, no relationship between AChE and total tissue protein was established in the anterior and posterior sections suggesting that the circannual rhythms observed are AChE-specific responses.Results highlight the importance of considering both the fish size and season variations to reach valid conclusions when AChE activity is employed as neurotoxicity biomarker. © 2014 Elsevier Inc. Fil:Menéndez-Helman, R.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferreyroa, G.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01476513_v111_n_p236_MenendezHelman |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acetylcholinesterase Biomarkers Circannual rhythms Cnesterodon decemmaculatus Seasonal variations acetylcholinesterase acetylcholinesterase biological marker fresh water water pollutant bioassay biomarker environmental risk freshwater environment pollution exposure protein seasonal variation teleost toxicity animal experiment animal tissue Article biological monitoring biological trait circannual rhythm Cnesterodon decemmaculatus controlled study ecotoxicology environmental impact assessment environmental stress enzyme activity enzyme mechanism fish length nonhuman physical chemistry physical parameters risk assessment seasonal variation summer teleost toxicity testing winter animal body size Cyprinodontiformes environmental monitoring metabolism photoperiodicity procedures season temperature toxicity water pollutant Cnesterodon decemmaculatus Acetylcholinesterase Animals Biological Markers Body Size Cyprinodontiformes Environmental Monitoring Fresh Water Photoperiod Seasons Temperature Water Pollutants, Chemical |
| spellingShingle |
Acetylcholinesterase Biomarkers Circannual rhythms Cnesterodon decemmaculatus Seasonal variations acetylcholinesterase acetylcholinesterase biological marker fresh water water pollutant bioassay biomarker environmental risk freshwater environment pollution exposure protein seasonal variation teleost toxicity animal experiment animal tissue Article biological monitoring biological trait circannual rhythm Cnesterodon decemmaculatus controlled study ecotoxicology environmental impact assessment environmental stress enzyme activity enzyme mechanism fish length nonhuman physical chemistry physical parameters risk assessment seasonal variation summer teleost toxicity testing winter animal body size Cyprinodontiformes environmental monitoring metabolism photoperiodicity procedures season temperature toxicity water pollutant Cnesterodon decemmaculatus Acetylcholinesterase Animals Biological Markers Body Size Cyprinodontiformes Environmental Monitoring Fresh Water Photoperiod Seasons Temperature Water Pollutants, Chemical Menéndez-Helman, R.J. Ferreyroa, G.V. dos Santos Afonso, M. Salibián, A. Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus |
| topic_facet |
Acetylcholinesterase Biomarkers Circannual rhythms Cnesterodon decemmaculatus Seasonal variations acetylcholinesterase acetylcholinesterase biological marker fresh water water pollutant bioassay biomarker environmental risk freshwater environment pollution exposure protein seasonal variation teleost toxicity animal experiment animal tissue Article biological monitoring biological trait circannual rhythm Cnesterodon decemmaculatus controlled study ecotoxicology environmental impact assessment environmental stress enzyme activity enzyme mechanism fish length nonhuman physical chemistry physical parameters risk assessment seasonal variation summer teleost toxicity testing winter animal body size Cyprinodontiformes environmental monitoring metabolism photoperiodicity procedures season temperature toxicity water pollutant Cnesterodon decemmaculatus Acetylcholinesterase Animals Biological Markers Body Size Cyprinodontiformes Environmental Monitoring Fresh Water Photoperiod Seasons Temperature Water Pollutants, Chemical |
| description |
The use of biomarkers as a tool to assess responses of organisms exposed to pollutants in toxicity bioassays, as well as in aquatic environmental risk assessment protocols, requires the understanding of the natural fluctuation of the particular biomarker. The aim of this study was to characterize the intrinsic variations of acetylcholinesterase (AChE) activity in tissues of a native freshwater teleost fish to be used as biomarker in toxicity tests, taking into account both seasonal influence and fish size.Specific AChE activity was measured by the method of Ellman et al. (1961) in homogenates of fish anterior section finding a seasonal variability. The highest activity was observed in summer, decreasing significantly below 40% in winter. The annual AChE activity cycle in the anterior section was fitted to a sinusoidal function with a period of 11.2 months. Moreover, an inverse relationship between enzymatic activity and the animal size was established. The results showed that both the fish length and seasonal variability affect AChE activity.AChE activity in fish posterior section showed a similar trend to that in the anterior section, while seasonal variations of the activity in midsection were observed but differences were not statistically significant.In addition, no relationship between AChE and total tissue protein was established in the anterior and posterior sections suggesting that the circannual rhythms observed are AChE-specific responses.Results highlight the importance of considering both the fish size and season variations to reach valid conclusions when AChE activity is employed as neurotoxicity biomarker. © 2014 Elsevier Inc. |
| format |
JOUR |
| author |
Menéndez-Helman, R.J. Ferreyroa, G.V. dos Santos Afonso, M. Salibián, A. |
| author_facet |
Menéndez-Helman, R.J. Ferreyroa, G.V. dos Santos Afonso, M. Salibián, A. |
| author_sort |
Menéndez-Helman, R.J. |
| title |
Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus |
| title_short |
Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus |
| title_full |
Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus |
| title_fullStr |
Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus |
| title_full_unstemmed |
Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus |
| title_sort |
circannual rhythms of acetylcholinesterase (ache) activity in the freshwater fish cnesterodon decemmaculatus |
| url |
http://hdl.handle.net/20.500.12110/paper_01476513_v111_n_p236_MenendezHelman |
| work_keys_str_mv |
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1807321221131403264 |