Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
Megaplatypus mutatus is a major forest pest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with a metagenomics approach (454-pyrosequencing). A total of 270 collection points from insect galleries from th...
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todo:paper_00375330_v52_n3_p_CerianiNakamurakare2023-10-03T14:48:07Z Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) Ceriani-Nakamurakare, E. Ramos, S. Robles, C.A. Novas, M.V. D’jonsiles, M.F. Gonzalez-Audino, P. Carmarán, C. Ambrosia Forest pest Fungi Insect-fungus interactions Metagenomic Platypodinae Cotton Forestry Location Ambrosia Forest pest Fungal community compositions Generalized linear mixed models Metagenomic Platypodinae Principal coordinates analysis Relative frequencies Fungi beetle community structure deciduous tree fungus genetic analysis genomics host plant host specificity species richness Argentina Casuarina Cotton Data Forestry Fungi Populus Deltoides Test Methods Argentina Europe Ambrosia Candida insectalens Casuarina cunninghamiana Coleoptera Fungi Fusarium Hexapoda Platypodini Populus deltoides Megaplatypus mutatus is a major forest pest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with a metagenomics approach (454-pyrosequencing). A total of 270 collection points from insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M. mutatus; compare the culture-independent approach with previous culturing studies, in terms of data recovery related to the fungal community composition, and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richness in each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analyses showed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6% of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus. © 2018, Finnish Society of Forest Science. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00375330_v52_n3_p_CerianiNakamurakare |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ambrosia Forest pest Fungi Insect-fungus interactions Metagenomic Platypodinae Cotton Forestry Location Ambrosia Forest pest Fungal community compositions Generalized linear mixed models Metagenomic Platypodinae Principal coordinates analysis Relative frequencies Fungi beetle community structure deciduous tree fungus genetic analysis genomics host plant host specificity species richness Argentina Casuarina Cotton Data Forestry Fungi Populus Deltoides Test Methods Argentina Europe Ambrosia Candida insectalens Casuarina cunninghamiana Coleoptera Fungi Fusarium Hexapoda Platypodini Populus deltoides |
| spellingShingle |
Ambrosia Forest pest Fungi Insect-fungus interactions Metagenomic Platypodinae Cotton Forestry Location Ambrosia Forest pest Fungal community compositions Generalized linear mixed models Metagenomic Platypodinae Principal coordinates analysis Relative frequencies Fungi beetle community structure deciduous tree fungus genetic analysis genomics host plant host specificity species richness Argentina Casuarina Cotton Data Forestry Fungi Populus Deltoides Test Methods Argentina Europe Ambrosia Candida insectalens Casuarina cunninghamiana Coleoptera Fungi Fusarium Hexapoda Platypodini Populus deltoides Ceriani-Nakamurakare, E. Ramos, S. Robles, C.A. Novas, M.V. D’jonsiles, M.F. Gonzalez-Audino, P. Carmarán, C. Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
| topic_facet |
Ambrosia Forest pest Fungi Insect-fungus interactions Metagenomic Platypodinae Cotton Forestry Location Ambrosia Forest pest Fungal community compositions Generalized linear mixed models Metagenomic Platypodinae Principal coordinates analysis Relative frequencies Fungi beetle community structure deciduous tree fungus genetic analysis genomics host plant host specificity species richness Argentina Casuarina Cotton Data Forestry Fungi Populus Deltoides Test Methods Argentina Europe Ambrosia Candida insectalens Casuarina cunninghamiana Coleoptera Fungi Fusarium Hexapoda Platypodini Populus deltoides |
| description |
Megaplatypus mutatus is a major forest pest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with a metagenomics approach (454-pyrosequencing). A total of 270 collection points from insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M. mutatus; compare the culture-independent approach with previous culturing studies, in terms of data recovery related to the fungal community composition, and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richness in each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analyses showed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6% of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus. © 2018, Finnish Society of Forest Science. All rights reserved. |
| format |
JOUR |
| author |
Ceriani-Nakamurakare, E. Ramos, S. Robles, C.A. Novas, M.V. D’jonsiles, M.F. Gonzalez-Audino, P. Carmarán, C. |
| author_facet |
Ceriani-Nakamurakare, E. Ramos, S. Robles, C.A. Novas, M.V. D’jonsiles, M.F. Gonzalez-Audino, P. Carmarán, C. |
| author_sort |
Ceriani-Nakamurakare, E. |
| title |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
| title_short |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
| title_full |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
| title_fullStr |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
| title_full_unstemmed |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
| title_sort |
metagenomic approach of associated fungi with megaplatypus mutatus (coleoptera: platypodinae) |
| url |
http://hdl.handle.net/20.500.12110/paper_00375330_v52_n3_p_CerianiNakamurakare |
| work_keys_str_mv |
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| _version_ |
1807315007752372224 |