Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america
Protoplasts were successfully isolated from internodal callus tissues of both Oxalis glaucifolia and O. rhombeo-ovata when they were digested in a solution containing 0.1% (w/v) Macerozyme R-10, 0.5% (w/v) cellulase Onozuka R-10 and 0.3 mmol m-3 sucrose. Protoplasts proliferated to give cell colonie...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00220957_v40_n4_p493_Ochatt |
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todo:paper_00220957_v40_n4_p493_Ochatt2023-10-03T14:26:12Z Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america Ochatt, S.J. Escandon, A.S. Martinez, A.J. Oxalis spp Plant regeneration Protoplasts Tissue culture Protoplasts were successfully isolated from internodal callus tissues of both Oxalis glaucifolia and O. rhombeo-ovata when they were digested in a solution containing 0.1% (w/v) Macerozyme R-10, 0.5% (w/v) cellulase Onozuka R-10 and 0.3 mmol m-3 sucrose. Protoplasts proliferated to give cell colonies on Gamborg et al.'s B5 medium supplemented with 0.3 mmol m-3 mannitol, 0.5 mg dm-32, 4-D, and 2.0 mg dm-3 kinetin. Callus was produced upon transfer of cell colonies to Murashige and Skoog medium containing 2.0 mg dm-3 l-naphthaleneacetic acid (NAA) and 0.1 mg dm-3 kinetin for O. glaucifolia, or with 5.0 mg dm-3 NAA and 0.5 mg dm-3 6-benzylaminopurine, for O. rhombeo-ovata. Plants were regenerated from O. glaucifolia protoplasts on a medium containing 0.1 mg dm-3 NAA, 1.0 mg dm-3 kinetin and 1.0 mg dm-3 gibberellic acid, but only vascular nodules were differentiated by O. rhombeo-ovata protoplast-derived calli. © 1989 Oxford University Press. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220957_v40_n4_p493_Ochatt |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Oxalis spp Plant regeneration Protoplasts Tissue culture |
| spellingShingle |
Oxalis spp Plant regeneration Protoplasts Tissue culture Ochatt, S.J. Escandon, A.S. Martinez, A.J. Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america |
| topic_facet |
Oxalis spp Plant regeneration Protoplasts Tissue culture |
| description |
Protoplasts were successfully isolated from internodal callus tissues of both Oxalis glaucifolia and O. rhombeo-ovata when they were digested in a solution containing 0.1% (w/v) Macerozyme R-10, 0.5% (w/v) cellulase Onozuka R-10 and 0.3 mmol m-3 sucrose. Protoplasts proliferated to give cell colonies on Gamborg et al.'s B5 medium supplemented with 0.3 mmol m-3 mannitol, 0.5 mg dm-32, 4-D, and 2.0 mg dm-3 kinetin. Callus was produced upon transfer of cell colonies to Murashige and Skoog medium containing 2.0 mg dm-3 l-naphthaleneacetic acid (NAA) and 0.1 mg dm-3 kinetin for O. glaucifolia, or with 5.0 mg dm-3 NAA and 0.5 mg dm-3 6-benzylaminopurine, for O. rhombeo-ovata. Plants were regenerated from O. glaucifolia protoplasts on a medium containing 0.1 mg dm-3 NAA, 1.0 mg dm-3 kinetin and 1.0 mg dm-3 gibberellic acid, but only vascular nodules were differentiated by O. rhombeo-ovata protoplast-derived calli. © 1989 Oxford University Press. |
| format |
JOUR |
| author |
Ochatt, S.J. Escandon, A.S. Martinez, A.J. |
| author_facet |
Ochatt, S.J. Escandon, A.S. Martinez, A.J. |
| author_sort |
Ochatt, S.J. |
| title |
Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america |
| title_short |
Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america |
| title_full |
Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america |
| title_fullStr |
Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america |
| title_full_unstemmed |
Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america |
| title_sort |
isolation, culture, and plant regeneration of protoplasts of two shrubby oxalis species from south america |
| url |
http://hdl.handle.net/20.500.12110/paper_00220957_v40_n4_p493_Ochatt |
| work_keys_str_mv |
AT ochattsj isolationcultureandplantregenerationofprotoplastsoftwoshrubbyoxalisspeciesfromsouthamerica AT escandonas isolationcultureandplantregenerationofprotoplastsoftwoshrubbyoxalisspeciesfromsouthamerica AT martinezaj isolationcultureandplantregenerationofprotoplastsoftwoshrubbyoxalisspeciesfromsouthamerica |
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1807318731200659456 |