Cellular proliferation rate and insulin-like growth factor binding protein (IGFBP)-2 and IGFBP-3 and estradiol receptor alpha expression in the mammary gland of dairy heifers naturally infected with gastrointestinal nematodes during development

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Mammary ductal morphogenesis during prepuberty occurs mainly in response to insulin-like growth factor-1 (IGF-1) and estradiol stimulation. Dairy heifers infected with gastrointestinal nematodes have reduced IGF-1 levels, accompanied by reduced growth rate, delayed puberty onset, and lower parenchym...

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Detalles Bibliográficos
Autores principales: Perri, A.F., Dallard, B.E., Baravalle, C., Licoff, N., Formía, N., Ortega, H.H., Becú-Villalobos, D., Mejia, M.E., Lacau-Mengido, I.M.
Formato: JOUR
Materias:
Estradiol receptor alpha
Insulin-like growth factor binding protein
Prepubertal mammary gland
Proliferating cell nuclear antigen
anthelmintic agent
estradiol
estrogen receptor alpha
fenbendazole
ivermectin
levamisole
somatomedin binding protein 2
somatomedin binding protein 3
somatomedin C
animal
bovine
cell proliferation
controlled study
cytology
female
gastrointestinal tract
genetics
metabolism
nematode
nematodiasis
parasitology
randomized controlled trial
signal transduction
udder
veterinary
Animals
Anthelmintics
Cattle
Cell Proliferation
Estradiol
Estrogen Receptor alpha
Female
Fenbendazole
Gastrointestinal Tract
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor I
Ivermectin
Levamisole
Mammary Glands, Animal
Nematoda
Nematode Infections
Signal Transduction
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00220302_v97_n8_p4985_Perri
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Mammary ductal morphogenesis during prepuberty occurs mainly in response to insulin-like growth factor-1 (IGF-1) and estradiol stimulation. Dairy heifers infected with gastrointestinal nematodes have reduced IGF-1 levels, accompanied by reduced growth rate, delayed puberty onset, and lower parenchyma-stroma relationship in their mammary glands. Immunohistochemical studies were undertaken to determine variations in cell division rate, IGF-1 system components, and estradiol receptors (ESR) during peripubertal development in the mammary glands of antiparasitic-treated and untreated Holstein heifers naturally infected with gastrointestinal nematodes. Mammary biopsies were taken at 20, 30, 40, and 70 wk of age. Proliferating cell nuclear antigen immunolabeling, evident in nuclei, tended to be higher in the parenchyma of the glands from treated heifers than in those from untreated. Insulin-like growth factor binding proteins (IGFBP) type 2 and type 3 immunolabeling was cytoplasmic and was evident in stroma and parenchyma. The IGFBP2-labeled area was lower in treated than in untreated heifers. In the treated group, a maximal expression of this protein was seen at 40 wk of age, whereas in the untreated group the labeling remained constant. No differences were observed for IGFBP3 between treatment groups or during development. Immunolabeling for α ESR (ESR1) was evident in parenchymal nuclei and was higher in treated than in untreated heifers. In the treated group, ESR1 peaked at 30 wk of age and then decreased. These results demonstrate that the parasite burden in young heifers negatively influence mammary gland development, affecting cell division rate and parameters related to estradiol and IGF-1 signaling in the gland. © 2014 American Dairy Science Association.

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