Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes
The effect of different parameters on the interfacial polarity of phospholipid vesicles was studied using two polarity-sensitive fluorescent probes, dansyldihexadecylamine (DA) and dansylphosphatidylethanolamine (DPE). The analysis was based on the Stokes shift and steady-state fluorescence anisotro...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219797_v203_n1_p97_Bernik |
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todo:paper_00219797_v203_n1_p97_Bernik2023-10-03T14:25:09Z Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes Bernik, D.L. Martín Negri, R. Dansyl probes Interfaces Liposomes Micropolarity Stokes shift dansyl chloride dansyldihexadecylamine dansylphosphatidylethanolamine liposome unclassified drug anisotropy article fluorescence hydration lipid composition lipid vesicle priority journal temperature The effect of different parameters on the interfacial polarity of phospholipid vesicles was studied using two polarity-sensitive fluorescent probes, dansyldihexadecylamine (DA) and dansylphosphatidylethanolamine (DPE). The analysis was based on the Stokes shift and steady-state fluorescence anisotropy changes with different parameters. The influence of surface curvature, lipid composition, and surface charge density was analyzed in small and large unilamellar vesicles and in multilamellar vesicles at different temperatures and in different lipid phase states. The membranes showed significant polarity, packing constraints, and hydration degree changes at the polar head level when different variables were imposed. The comparative analysis of the probe response demonstrates that each dansyl derivative yields different information about membrane changes. DA appears to be more sensitive to packing constraints and hydration, probably due to its molecular structure and location at the interface level. DPE seems to sense polarity values averaged in the region where its polar head can pivot, and is, as expected, susceptible to electrostatic interactions. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219797_v203_n1_p97_Bernik |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Dansyl probes Interfaces Liposomes Micropolarity Stokes shift dansyl chloride dansyldihexadecylamine dansylphosphatidylethanolamine liposome unclassified drug anisotropy article fluorescence hydration lipid composition lipid vesicle priority journal temperature |
| spellingShingle |
Dansyl probes Interfaces Liposomes Micropolarity Stokes shift dansyl chloride dansyldihexadecylamine dansylphosphatidylethanolamine liposome unclassified drug anisotropy article fluorescence hydration lipid composition lipid vesicle priority journal temperature Bernik, D.L. Martín Negri, R. Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes |
| topic_facet |
Dansyl probes Interfaces Liposomes Micropolarity Stokes shift dansyl chloride dansyldihexadecylamine dansylphosphatidylethanolamine liposome unclassified drug anisotropy article fluorescence hydration lipid composition lipid vesicle priority journal temperature |
| description |
The effect of different parameters on the interfacial polarity of phospholipid vesicles was studied using two polarity-sensitive fluorescent probes, dansyldihexadecylamine (DA) and dansylphosphatidylethanolamine (DPE). The analysis was based on the Stokes shift and steady-state fluorescence anisotropy changes with different parameters. The influence of surface curvature, lipid composition, and surface charge density was analyzed in small and large unilamellar vesicles and in multilamellar vesicles at different temperatures and in different lipid phase states. The membranes showed significant polarity, packing constraints, and hydration degree changes at the polar head level when different variables were imposed. The comparative analysis of the probe response demonstrates that each dansyl derivative yields different information about membrane changes. DA appears to be more sensitive to packing constraints and hydration, probably due to its molecular structure and location at the interface level. DPE seems to sense polarity values averaged in the region where its polar head can pivot, and is, as expected, susceptible to electrostatic interactions. |
| format |
JOUR |
| author |
Bernik, D.L. Martín Negri, R. |
| author_facet |
Bernik, D.L. Martín Negri, R. |
| author_sort |
Bernik, D.L. |
| title |
Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes |
| title_short |
Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes |
| title_full |
Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes |
| title_fullStr |
Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes |
| title_full_unstemmed |
Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes |
| title_sort |
local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes |
| url |
http://hdl.handle.net/20.500.12110/paper_00219797_v203_n1_p97_Bernik |
| work_keys_str_mv |
AT bernikdl localpolarityatthepolarheadleveloflipidvesiclesusingdansylfluorescentprobes AT martinnegrir localpolarityatthepolarheadleveloflipidvesiclesusingdansylfluorescentprobes |
| _version_ |
1807324172446072832 |