Interleukin-1β enhances interleukin-1 receptor antagonist content in human somatotroph adenoma cell cultures

In addition to the well-known modulation of immune and inflammatory responses, the interleukin-1 (IL-1) system has been shown to be involved in the regulation of anterior pituitary hormone secretion and growth. We previously demonstrated that IL-1 receptor antagonist (IL-1ra) is expressed in human p...

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Detalles Bibliográficos
Autores principales: Sauer, J., Renner, U., Hopfner, U., Lange, M., Müller, A., Strasburger, C.J., Pagotto, U., Arzt, E., Stalla, G.K.
Formato: JOUR
Materias:
growth hormone
interleukin 1 receptor blocking agent
interleukin 1beta
adult
aged
article
cell culture
cell growth
clinical article
concentration response
cytokine release
female
gene expression regulation
growth hormone blood level
growth hormone release
hormone release
human
human tissue
hypophysis adenoma
male
priority journal
somatic cell
supernatant
tissue level
Adenoma
Adult
Aged
Cells, Cultured
Female
Humans
Interleukin-1
Male
Middle Aged
Monocytes
Pituitary Neoplasms
Receptors, Interleukin-1
Tumor Cells, Cultured
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0021972X_v83_n7_p2429_Sauer
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:In addition to the well-known modulation of immune and inflammatory responses, the interleukin-1 (IL-1) system has been shown to be involved in the regulation of anterior pituitary hormone secretion and growth. We previously demonstrated that IL-1 receptor antagonist (IL-1ra) is expressed in human pituitary adenomas cultured in vitro. In the present study, we investigated the regulation of IL-1ra protein by IL-1β (1-100 U/mL) in human somatotroph adenomas (n = 9) cultured for 12-48 h. IL-1β significantly enhanced the concentration of IL-1ra dose dependently in the somatotroph adenoma cell lysates, whereas IL-1ra concentrations remained unchanged in the culture supernatants. Furthermore, basal IL-1ra concentrations were significantly higher in the cell lysates compared with the corresponding culture supernatants. The regulation of IL-1ra in somatotroph adenoma cells is different from human cultured monocytes, in which IL-1β significantly stimulated IL-1ra secretion into the culture supernatants, and no change of intracellular IL-1ra content was observed. Incubation of the somatotroph adenoma cells with 100 U/mL IL-1β did not result in a change of GH concentrations in the culture supernatants. Enhancement of intracellular IL- 1ra protein by IL-1β may represent a mechanism intrinsic to somatotroph adenoma cells to counterregulate the response to IL-1β on hormone secretion or cellular growth.

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