A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii

Background: Rhomboid proteases are ubiquitous, and their role in Archaea has not been explored. Results: We generated a rhomboid deletion mutant that displayed a glycosylation defect. Conclusion: Deletion of a rhomboid protease gene altered S-layer glycoprotein N-glycosylation. Significance: This wo...

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Detalles Bibliográficos
Autores principales: Parente, J., Casabuono, A., Ferrari, M.C., Paggi, R.A., De Castro, R.E., Couto, A.S., Giménez, M.I.
Formato: JOUR
Materias:
Esterification
Genes
Glycosylation
Oligosaccharides
Deletion mutants
Gene deletion
Haloferax volcanii
N-Glycosylation
N-linked
Protein glycosylation
S-layers
Structural characterization
Glycoproteins
glycoprotein
oligosaccharide
proteinase
RhoII protease
unclassified drug
article
bacterial gene
bacterial strain
bacterium mutant
carbohydrate analysis
controlled study
enzyme activity
gene deletion
high performance liquid chromatography
mass spectrometry
nonhuman
priority journal
protein glycosylation
RhoII gene
structure analysis
Archaea
Glycoprotein
Halophiles
Mass Spectrometry (MS)
Rhomboid Protease
Endopeptidases
Gene Knockdown Techniques
Membrane Glycoproteins
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00219258_v289_n16_p11304_Parente
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Background: Rhomboid proteases are ubiquitous, and their role in Archaea has not been explored. Results: We generated a rhomboid deletion mutant that displayed a glycosylation defect. Conclusion: Deletion of a rhomboid protease gene altered S-layer glycoprotein N-glycosylation. Significance: This work provides structural characterization of a novel oligosaccharide bound to H. volcanii S-layer glycoprotein and relates a rhomboid protease with the protein glycosylation process. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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