Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 16...
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| Autores principales: | , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219193_v168_n1_p270_Ugalde |
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| Sumario: | Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated glactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end. |
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