Enzyme replacement therapy in porphyrias-III: Potential use of erythrocyte ghosts as carriers of δ-aminolaevulinate dehydratase
1. 1. Resealed erythrocytes ghosts are potential in vivo carriers of exogenous therapeutic enzymes. The present work is part of studies directed toward the use of enzymes encapsulated within artificial or natural cellular carriers, in enzyme replacement therapy in porphyrias. 2. 2. Lead intoxication...
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| Autores principales: | , , , |
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| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0020711X_v15_n3_p447_Bustos |
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| Sumario: | 1. 1. Resealed erythrocytes ghosts are potential in vivo carriers of exogenous therapeutic enzymes. The present work is part of studies directed toward the use of enzymes encapsulated within artificial or natural cellular carriers, in enzyme replacement therapy in porphyrias. 2. 2. Lead intoxication was chosen as a model system for these experiments. 3. 3. The optimum conditions for both the preparation of erythrocyte ghosts and encapsulation of δ-aminolaevulinate dehydratase (ALA-D) were studied in detail and a very good yield procedure for entrapment of exogenous highly purified human blood ALA-D, into both normal and defective erythrocytes was developed. 4. 4. The capacity of ALA-D defective cells of lead intoxicated patients and animals was brought within the normal range, in vitro, either by introducing ALA-D into them or by mixing with ALA-D loaded erythrocyte ghosts. 5. 5. Red blood cell ghosts entrapment is therefore a promising approach to the delivery of ALA-D when applying enzyme replacement therapy in lead poisoning. © 1983. |
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