Occurrence of multiple molecular forms of porphobilinogenase in diverse organisms: The minimum quaternary structure of porphobilinogenase is a protomer of one deaminase and one isomerase domain

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1. 1. Gel filtration on Sephadex G-100 and Sepharose 4B were used to redetermine the molecular weight (MW) of porphobilinogenase, deaminase and isomerase purified from different sources, and determine the MW of these enzymes purified from Eugtena gracilis. 2. 2. Results reported here, indicate that...

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Detalles Bibliográficos
Autores principales: Rossetti, M.V., de Geralnik, A.A.J., Kotler, M., Fumagalli, S., del C. Batlle, A.M.
Formato: JOUR
Materias:
ammonia lyase
Ammonia Lyases
hydrolase
isomerase
multienzyme complex
animal
article
bird
cattle
comparative study
enzymology
erythrocyte
Euglena gracilis
isolation and purification
liver
macromolecule
molecular weight
plant
protein conformation
species difference
Aminohydrolases
Ammonia-Lyases
Animal
Birds
Cattle
Comparative Study
Erythrocytes
Isomerases
Liver
Macromolecular Systems
Molecular Weight
Multienzyme Complexes
Plants
Protein Conformation
Species Specificity
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p761_Rossetti
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:1. 1. Gel filtration on Sephadex G-100 and Sepharose 4B were used to redetermine the molecular weight (MW) of porphobilinogenase, deaminase and isomerase purified from different sources, and determine the MW of these enzymes purified from Eugtena gracilis. 2. 2. Results reported here, indicate that porphobilinogenase can be found, into three different molecular forms, tetramers, dimers and monomers according to the source organism. 3. 3. It is proposed that minimal functional structure of PBGase is a hybrid protomer of MW 25,000, composed by two different domains, in a ratio of 1 mol of deaminase, MW 20,000 to 1 mol of isomerase. MW 5000. 4. 4. A model explaining the occurrence of different MW species of PBGase in nature and the possible interconversion among the various forms is postulated. © 1980.

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