Characterization of a sexual steroid binding protein in Bufo arenarum

Reproductively active female Bufo arenarum possess a serum protein (SBP) that binds testosterone, dihydrotestosterone, and estradiol with high affinity and capacity. The binding capacity values are two orders of magnitude higher than those found in human serum. The specificity of B. arenarum SBP is...

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Detalles Bibliográficos
Autores principales: Santa-Coloma, T.A., Fernandez, S., Charreau, E.H.
Formato: JOUR
Materias:
androstanolone
estradiol
metribolone
sex hormone binding protein
testosterone
endocrine system
female genital system
male genital system
methodology
molecular weight
nonhuman
priority journal
sedimentation coefficient
Animal
Bufo arenarum
Electrophoresis, Polyacrylamide Gel
Estradiol
Kinetics
Sex Hormone-Binding Globulin
Stanolone
Substrate Specificity
Testosterone
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00166480_v60_n2_p273_SantaColoma
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Reproductively active female Bufo arenarum possess a serum protein (SBP) that binds testosterone, dihydrotestosterone, and estradiol with high affinity and capacity. The binding capacity values are two orders of magnitude higher than those found in human serum. The specificity of B. arenarum SBP is comparable to human SBP with the exception that it has a high affinity for the synthetic androgen methyltrienolone (R1881). The physicochemical characterization of SBP indicates a Stokes radius of 43.5 Å, a sedimentation coefficient of 5.2 S, and a molecular weight of 93,300. © 1985.

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