Binding and effects of catecholestrogens on adenylate cyclase activity, and adrenoceptors, benzodiazepine and GABA receptors in guinea-pig hypothalamic membranes

Catecholestrogen (CE) binding to guinea-pig hypothalamic membranes was assessed by using [3H]2-hydroxyestrone (2-OHE2) as a ligand. Binding was maximal at pH 7.4 and 37°C, and after 10 min incubations. A high affinity binding site with dissociation constant (KD) = 0.20 ± 0.02 nM and site concentrati...

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Detalles Bibliográficos
Autores principales: Etchegoyen, G.S., Cardinali, D.P., Pérez, A.E., Tamayo, J., Pérez-Palacios, G.
Formato: JOUR
Materias:
Adenylate cyclase
Adrenoceptor binding
Benzodiazepine binding
Catecholestrogens
GABAergic binding
Hypothalamus
2 hydroxyestradiol
2 hydroxyestrone
2 hydroxyestrone h 3
4 aminobutyric acid h 3
4 aminobutyric acid receptor
adenylate cyclase
adrenalin
alpha adrenergic receptor
benzodiazepine receptor
beta adrenergic receptor
catechol estrogen
dihydroalprenolol h 3
dihydroergocryptine h 3
domperidone
dopamine
estradiol
estrone
flunitrazepam h 3
noradrenalin
phentolamine
propranolol
radioisotope
unclassified drug
animal cell
article
central nervous system
drug binding
drug receptor binding
guinea pig
hypothalamus
nervous system
nonhuman
pharmacokinetics
priority journal
synaptosome
Adenylate Cyclase
Animals
Binding, Competitive
Cell Membrane
Enzyme Activation
Estrogens, Catechol
Guinea Pigs
Norepinephrine
Receptors, Adrenergic
Receptors, GABA-A
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00142999_v129_n1-2_p1_Etchegoyen
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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