Expression of human proacrosin in Escherichia coli and binding to zona pellucida
Proacrosin is a multifunctional protein present in the sperm acrosome. This study characterizes the expression of human proacrosin in bacteria and assesses zona pellucida binding activity. The cDNA encoding human proacrosin was subcloned in pGEX-3X and pET-22b vectors. In the pGEX system, expression...
| Autores principales: | Furlong, L.I., Hellman, U., Krimer, A., Tezón, J.G., Charreau, E.H., Vazquez-Levin, M.H. |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00063363_v62_n3_p606_Furlong |
| Aporte de: |
Ejemplares similares
-
Expression of human proacrosin in Escherichia coli and binding to zona pellucida
Publicado: (2000) -
Immobilized escherichia coli BL21 as a catalyst for the synthesis of adenine and hypoxanthine nucleosides
Publicado: (2004) -
Immobilized escherichia coli BL21 as a catalyst for the synthesis of adenine and hypoxanthine nucleosides
por: Trelles, J.A., et al. -
Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
Publicado: (1998) -
Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
por: Pereira, C.M., et al.