Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells
Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are a promising source of cells for regenerative medicine because of their potential of self renew and differentiation. Multiple evidences highlight the relationship of chromatin remodeling with stem cell properties, differentiat...
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todo:paper_0006291X_v410_n4_p816_Luzzani2023-10-03T14:03:59Z Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells Luzzani, C. Solari, C. Losino, N. Ariel, W. Romorini, L. Bluguermann, C. Sevlever, G. Barañao, L. Miriuka, S. Guberman, A. Chromatin modifying factors Differentiation Embryonic stem cells Induced pluripotent stem cells BMI1 protein cell protein histone deacetylase histone deacetylase 10 histone deacetylase 2 histone deacetylase 3 histone deacetylase 5 protein atf7ip protein gcn512 protein gtf3c3 protein Jarid2 protein myst2 protein suv39h2 protein taf15 unclassified drug animal cell article cell differentiation chromatin assembly and disassembly embryonic stem cell gene expression human human cell mouse nonhuman pluripotent stem cell priority journal protein expression Animals Cell Line Chromatin Chromatin Immunoprecipitation Embryonic Stem Cells Gene Expression Regulation Humans Induced Pluripotent Stem Cells Mice Transcription Factors Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are a promising source of cells for regenerative medicine because of their potential of self renew and differentiation. Multiple evidences highlight the relationship of chromatin remodeling with stem cell properties, differentiation programs and reprogramming for iPSC obtention.With the purpose of finding chromatin modifying factors relevant to these processes, and based on ChIP on chip studies, we selected several genes that could be modulated by Oct4, Sox2 and Nanog, critical transcription factors in stem cells, and studied their expression profile along the differentiation in mouse and human ESCs, and in mouse iPSCs. In this work, we analyzed the expression of Gcn5l2, GTF3C3, TAF15, ATF7IP, Myst2, HDAC2, HDAC3, HDAC5, HDAC10, SUV39H2, Jarid2, and Bmi-1. We found some genes from different functional groups that were highly modulated, suggesting that they could be relevant both in the undifferentiated state and during differentiation. These findings could contribute to the comprehension of molecular mechanisms involved in pluripotency, early differentiation and reprogramming. We believe that a deeper knowledge of the epigenetic regulation of ESC will allow improving somatic cell reprogramming for iPSC obtention and differentiation protocols optimization. © 2011 Elsevier Inc. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Losino, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romorini, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n4_p816_Luzzani |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chromatin modifying factors Differentiation Embryonic stem cells Induced pluripotent stem cells BMI1 protein cell protein histone deacetylase histone deacetylase 10 histone deacetylase 2 histone deacetylase 3 histone deacetylase 5 protein atf7ip protein gcn512 protein gtf3c3 protein Jarid2 protein myst2 protein suv39h2 protein taf15 unclassified drug animal cell article cell differentiation chromatin assembly and disassembly embryonic stem cell gene expression human human cell mouse nonhuman pluripotent stem cell priority journal protein expression Animals Cell Line Chromatin Chromatin Immunoprecipitation Embryonic Stem Cells Gene Expression Regulation Humans Induced Pluripotent Stem Cells Mice Transcription Factors |
| spellingShingle |
Chromatin modifying factors Differentiation Embryonic stem cells Induced pluripotent stem cells BMI1 protein cell protein histone deacetylase histone deacetylase 10 histone deacetylase 2 histone deacetylase 3 histone deacetylase 5 protein atf7ip protein gcn512 protein gtf3c3 protein Jarid2 protein myst2 protein suv39h2 protein taf15 unclassified drug animal cell article cell differentiation chromatin assembly and disassembly embryonic stem cell gene expression human human cell mouse nonhuman pluripotent stem cell priority journal protein expression Animals Cell Line Chromatin Chromatin Immunoprecipitation Embryonic Stem Cells Gene Expression Regulation Humans Induced Pluripotent Stem Cells Mice Transcription Factors Luzzani, C. Solari, C. Losino, N. Ariel, W. Romorini, L. Bluguermann, C. Sevlever, G. Barañao, L. Miriuka, S. Guberman, A. Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells |
| topic_facet |
Chromatin modifying factors Differentiation Embryonic stem cells Induced pluripotent stem cells BMI1 protein cell protein histone deacetylase histone deacetylase 10 histone deacetylase 2 histone deacetylase 3 histone deacetylase 5 protein atf7ip protein gcn512 protein gtf3c3 protein Jarid2 protein myst2 protein suv39h2 protein taf15 unclassified drug animal cell article cell differentiation chromatin assembly and disassembly embryonic stem cell gene expression human human cell mouse nonhuman pluripotent stem cell priority journal protein expression Animals Cell Line Chromatin Chromatin Immunoprecipitation Embryonic Stem Cells Gene Expression Regulation Humans Induced Pluripotent Stem Cells Mice Transcription Factors |
| description |
Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are a promising source of cells for regenerative medicine because of their potential of self renew and differentiation. Multiple evidences highlight the relationship of chromatin remodeling with stem cell properties, differentiation programs and reprogramming for iPSC obtention.With the purpose of finding chromatin modifying factors relevant to these processes, and based on ChIP on chip studies, we selected several genes that could be modulated by Oct4, Sox2 and Nanog, critical transcription factors in stem cells, and studied their expression profile along the differentiation in mouse and human ESCs, and in mouse iPSCs. In this work, we analyzed the expression of Gcn5l2, GTF3C3, TAF15, ATF7IP, Myst2, HDAC2, HDAC3, HDAC5, HDAC10, SUV39H2, Jarid2, and Bmi-1. We found some genes from different functional groups that were highly modulated, suggesting that they could be relevant both in the undifferentiated state and during differentiation. These findings could contribute to the comprehension of molecular mechanisms involved in pluripotency, early differentiation and reprogramming. We believe that a deeper knowledge of the epigenetic regulation of ESC will allow improving somatic cell reprogramming for iPSC obtention and differentiation protocols optimization. © 2011 Elsevier Inc. |
| format |
JOUR |
| author |
Luzzani, C. Solari, C. Losino, N. Ariel, W. Romorini, L. Bluguermann, C. Sevlever, G. Barañao, L. Miriuka, S. Guberman, A. |
| author_facet |
Luzzani, C. Solari, C. Losino, N. Ariel, W. Romorini, L. Bluguermann, C. Sevlever, G. Barañao, L. Miriuka, S. Guberman, A. |
| author_sort |
Luzzani, C. |
| title |
Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells |
| title_short |
Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells |
| title_full |
Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells |
| title_fullStr |
Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells |
| title_full_unstemmed |
Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells |
| title_sort |
modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n4_p816_Luzzani |
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1807316968790818816 |