The reactivation of stationary phase ribosomes from Bacillus stearothermophilus

Ribosomes from stationary phase Bacillus stearothermophilus have a diminished activity in protein synthesis due to the presence of a bound protein (association factor I, AFI). This factor inhibits the binding of fmet-tRNA to ribosomes. The inhibition of protein synthesis can be reversed in systems d...

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Guardado en:
Detalles Bibliográficos
Autor principal: García-Patrone, M.
Formato: JOUR
Materias:
animal experiment
bacillus stearothermophilus
in vitro study
ribosome
Bacillus stearothermophilus
Bacteriophages
Cell Cycle
Hypertonic Solutions
Peptide Initiation Factors
Poly U
Proteins
Ribosomal Proteins
Ribosomes
RNA, Messenger
RNA, Viral
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v104_n4_p1413_GarciaPatrone
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Ribosomes from stationary phase Bacillus stearothermophilus have a diminished activity in protein synthesis due to the presence of a bound protein (association factor I, AFI). This factor inhibits the binding of fmet-tRNA to ribosomes. The inhibition of protein synthesis can be reversed in systems directed by viral mRNA by the addition of an excess of high salt ribosomal wash obtained from mid-log cells. Under this condition AFI is released from ribosomes. © 1982.

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