Rat harderian gland porphobilinogen deaminase: Characterization studies and regulatory action of protoporphyrin IX

Properties of purified porphobilinogen deaminase (PBG-D; EC 4.3.1.8) from rat harderian gland are here presented. The enzyme behaves as a monomer of M(r) 38 ± 2 kDa and is optimally active at pH 8.0-8.2. Its activation energy, determined by an Arrhenius plot, is 76.1 kJ/mol. Initial velocity studies...

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Detalles Bibliográficos
Autores principales: Cardalda, C.A., Juknat, A.A., Princ, F.G., Batlle, A.
Formato: JOUR
Materias:
Cysteine residue(s)
Porphobilinogen deaminase
Porphobilinogen deaminase regulation
Protoporphyrin IX
Rat harderian gland
5,5' dithiobis(2 nitrobenzoic acid)
cysteine
n ethylmaleimide
porphobilinogen deaminase
protoporphyrin
uroporphyrin
animal tissue
article
catalysis
enzyme activity
enzyme analysis
enzyme kinetics
enzyme purification
enzyme substrate
Harder gland
male
molecular weight
nonhuman
priority journal
rat
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00039861_v347_n1_p69_Cardalda
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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