The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii

Two heat-stable inhibitors (a and b) of phosphoprotein phosphatases I and II from Mucor rouxii were isolated from mycelium of the fungus. They were partially purified from extracts by heating, DEAE-cellulose chromatography, and Sephadex G-75 gel filtration. The molecular weights of inhibitors a and...

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Autores principales: Seigelchifer, M.A., Passeron, S.
Formato: JOUR
Materias:
enzyme inhibitor
phosphatase
phosphoprotein
fungus
mucor rouxii
nonhuman
priority journal
protein phosphorylation
Chemistry
Chromatography, Gel
Heat
Molecular Weight
Mucor
Phosphoprotein Phosphatase
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00039861_v238_n1_p353_Seigelchifer
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00039861_v238_n1_p353_Seigelchifer
record_format dspace
spelling todo:paper_00039861_v238_n1_p353_Seigelchifer2023-10-03T13:56:59Z The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii Seigelchifer, M.A. Passeron, S. enzyme inhibitor phosphatase phosphoprotein fungus mucor rouxii nonhuman priority journal protein phosphorylation Chemistry Chromatography, Gel Heat Molecular Weight Mucor Phosphoprotein Phosphatase Support, Non-U.S. Gov't Two heat-stable inhibitors (a and b) of phosphoprotein phosphatases I and II from Mucor rouxii were isolated from mycelium of the fungus. They were partially purified from extracts by heating, DEAE-cellulose chromatography, and Sephadex G-75 gel filtration. The molecular weights of inhibitors a and b, estimated by gel filtration, are 5,000 and 20,000 respectively. Inhibitor a acts similarly on both enzymes while inhibitor b is relatively more active on enzyme II. Storage of inhibitor b at -20 °C for several weeks resulted in a partial conversion to a lower-molecular-weight form with properties similar to those of inhibitor a. © 1985. Fil:Seigelchifer, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00039861_v238_n1_p353_Seigelchifer
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic enzyme inhibitor
phosphatase
phosphoprotein
fungus
mucor rouxii
nonhuman
priority journal
protein phosphorylation
Chemistry
Chromatography, Gel
Heat
Molecular Weight
Mucor
Phosphoprotein Phosphatase
Support, Non-U.S. Gov't
spellingShingle enzyme inhibitor
phosphatase
phosphoprotein
fungus
mucor rouxii
nonhuman
priority journal
protein phosphorylation
Chemistry
Chromatography, Gel
Heat
Molecular Weight
Mucor
Phosphoprotein Phosphatase
Support, Non-U.S. Gov't
Seigelchifer, M.A.
Passeron, S.
The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii
topic_facet enzyme inhibitor
phosphatase
phosphoprotein
fungus
mucor rouxii
nonhuman
priority journal
protein phosphorylation
Chemistry
Chromatography, Gel
Heat
Molecular Weight
Mucor
Phosphoprotein Phosphatase
Support, Non-U.S. Gov't
description Two heat-stable inhibitors (a and b) of phosphoprotein phosphatases I and II from Mucor rouxii were isolated from mycelium of the fungus. They were partially purified from extracts by heating, DEAE-cellulose chromatography, and Sephadex G-75 gel filtration. The molecular weights of inhibitors a and b, estimated by gel filtration, are 5,000 and 20,000 respectively. Inhibitor a acts similarly on both enzymes while inhibitor b is relatively more active on enzyme II. Storage of inhibitor b at -20 °C for several weeks resulted in a partial conversion to a lower-molecular-weight form with properties similar to those of inhibitor a. © 1985.
format JOUR
author Seigelchifer, M.A.
Passeron, S.
author_facet Seigelchifer, M.A.
Passeron, S.
author_sort Seigelchifer, M.A.
title The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii
title_short The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii
title_full The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii
title_fullStr The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii
title_full_unstemmed The presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, Mucor rouxii
title_sort presence of two heat-stable inhibitors of phosphoprotein phosphatases in the dimorphic fungus, mucor rouxii
url http://hdl.handle.net/20.500.12110/paper_00039861_v238_n1_p353_Seigelchifer
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AT passerons thepresenceoftwoheatstableinhibitorsofphosphoproteinphosphatasesinthedimorphicfungusmucorrouxii
AT seigelchiferma presenceoftwoheatstableinhibitorsofphosphoproteinphosphatasesinthedimorphicfungusmucorrouxii
AT passerons presenceoftwoheatstableinhibitorsofphosphoproteinphosphatasesinthedimorphicfungusmucorrouxii
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