A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis

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A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity pattern...

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Detalles Bibliográficos
Autores principales: Krisman, C.R., Blumenfeld, M.L.
Formato: JOUR
Materias:
Glycogen synthase assay
glycogen synthase gel electrophoresis
glycogen synthase polyacrylamide gel detection
iodine staining
α-1,4 glucan synthase autoradiography
α-1,4 glucan synthase iodine staining
glycogen synthase
enzyme assay
methodology
nonhuman
polyacrylamide gel electrophoresis
priority journal
alpha-Amylase
Animal
Autoradiography
Electrophoresis, Polyacrylamide Gel
Glycogen Synthase
Iodine
Myocardium
Rats
Solutions
Staining and Labeling
Support, Non-U.S. Gov't
Time Factors
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00032697_v154_n2_p409_Krisman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity patterns were obtained by autoradiography or iodine staining of the gels. The method here described offers several advantages in terms of speed, sensitivity, and economy when compared with other procedures. © 1986.

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