Assay of dolichol monophosphate and dolichol diphosphate phosphatases: Thin-layer chromatography in a nonequilibrated system

A simple and rapid method for determining the activity of dolichol monophosphate phosphatase and dolichol diphosphate phosphatase is reported. This procedure is based on the separation of the substrates, [3H]dolichol monophosphate and [3H]dolichol diphosphate, from their reaction products, [3H]dolic...

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Detalles Bibliográficos
Autores principales: Boscoboinik, D.O., Belocopitow, E.
Formato: JOUR
Materias:
dolichol phosphate
phosphatase
dolichol diphosphate
enzyme assay
methodology
thin layer chromatography
Animal
Chromatography, Thin Layer
Kinetics
Microsomes, Liver
Phosphoric Monoester Hydrolases
Pyrophosphatases
Radioisotope Dilution Technique
Rats
Support, Non-U.S. Gov't
Tritium
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00032697_v114_n1_p42_Boscoboinik
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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