Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples
A recently introduced redox osmium complex of the type [Os(bpy)2Cl(pyX]+, where pyX corresponds to a pyridine derivative bearing an aldehyde group, and glucose oxidase [EC 1.1.3.4] were covalently immobilized on the glassy carbon electrode surface (upper cell body). This approach has been designed i...
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| Autores principales: | , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00032670_v443_n1_p17_Lapierre |
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| Sumario: | A recently introduced redox osmium complex of the type [Os(bpy)2Cl(pyX]+, where pyX corresponds to a pyridine derivative bearing an aldehyde group, and glucose oxidase [EC 1.1.3.4] were covalently immobilized on the glassy carbon electrode surface (upper cell body). This approach has been designed in a sealed cell to be used under continuous-flow/stopped-flow/continuous-flow processing. The operating characteristic of the integrated bioreactor/detector unit has been utilized in the determination of glucose in serum samples using the electroactive polymer as mediator in the re-oxidation of GOx and amperometric monitoring. The determinations are based on the rate of response under stopped-flow conditions. The trend in values of the apparent Michaelis-Menten constant confirms the validity of the approach to sensitive determination utilizing very small amounts of biocatalysts. The determination of glucose was possible with a limit of detection of 0.020 mM in the processing of as many as 30 samples per hour. © 2001 Elsevier Science B.V. All rights reserved. |
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