Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation

We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregatio...

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Detalles Bibliográficos
Autores principales: Yushchenko, D.A., Fauerbach, J.A., Thirunavukkuarasu, S., Jares-Erijman, E.A., Jovin, T.M.
Formato: JOUR
Materias:
Continuous monitoring
Dual emissions
Fluorescent probes
Intermediate stage
Parkinsons disease
Protein aggregation
Ratiometric
Sensor molecules
Synuclein
Probes
Fluorescence
acetylcysteine
alanine
alpha synuclein
amyloid
chromone derivative
cysteine
maleimide
thiol
article
fluorescence spectroscopy
molecular sensor
protein aggregation
proton transport
synthesis
alpha-Synuclein
Fluorescent Dyes
Maleimides
Models, Molecular
Protein Multimerization
Protein Structure, Quaternary
Spectrometry, Fluorescence
Sulfhydryl Compounds
Time Factors
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00027863_v132_n23_p7860_Yushchenko
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease. © 2010 American Chemical Society.

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