Analysis of persistence during intracellular actin-based transport mediated by molecular motors

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The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the mot...

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Autores principales: Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L.
Formato: Documento de conferencia publishedVersion
Lenguaje:Inglés
Publicado: 2010
Materias:
Cytology
Anomalous diffusion
Cell cytoplasm
Function of time
Molecular motors
Spatio-temporal resolution
Tracking techniques
Turning angles
Xenopus laevis
Proteins
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_17426588_v246_n_p_Pallavicini
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paperaa:paper_17426588_v246_n_p_Pallavicini
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spelling paperaa:paper_17426588_v246_n_p_Pallavicini2023-06-12T16:51:01Z Analysis of persistence during intracellular actin-based transport mediated by molecular motors J. Phys. Conf. Ser. 2010;246 Pallavicini, C. Despósito, M.A. Levi, V. Bruno, L. Cytology Anomalous diffusion Cell cytoplasm Function of time Molecular motors Spatio-temporal resolution Tracking techniques Turning angles Xenopus laevis Proteins The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd. Fil:Despósito, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 info:eu-repo/semantics/conferenceObject info:ar-repo/semantics/documento de conferencia info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_17426588_v246_n_p_Pallavicini
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic Cytology
Anomalous diffusion
Cell cytoplasm
Function of time
Molecular motors
Spatio-temporal resolution
Tracking techniques
Turning angles
Xenopus laevis
Proteins
spellingShingle Cytology
Anomalous diffusion
Cell cytoplasm
Function of time
Molecular motors
Spatio-temporal resolution
Tracking techniques
Turning angles
Xenopus laevis
Proteins
Pallavicini, C.
Despósito, M.A.
Levi, V.
Bruno, L.
Analysis of persistence during intracellular actin-based transport mediated by molecular motors
topic_facet Cytology
Anomalous diffusion
Cell cytoplasm
Function of time
Molecular motors
Spatio-temporal resolution
Tracking techniques
Turning angles
Xenopus laevis
Proteins
description The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd.
format Documento de conferencia
Documento de conferencia
publishedVersion
author Pallavicini, C.
Despósito, M.A.
Levi, V.
Bruno, L.
author_facet Pallavicini, C.
Despósito, M.A.
Levi, V.
Bruno, L.
author_sort Pallavicini, C.
title Analysis of persistence during intracellular actin-based transport mediated by molecular motors
title_short Analysis of persistence during intracellular actin-based transport mediated by molecular motors
title_full Analysis of persistence during intracellular actin-based transport mediated by molecular motors
title_fullStr Analysis of persistence during intracellular actin-based transport mediated by molecular motors
title_full_unstemmed Analysis of persistence during intracellular actin-based transport mediated by molecular motors
title_sort analysis of persistence during intracellular actin-based transport mediated by molecular motors
publishDate 2010
url http://hdl.handle.net/20.500.12110/paper_17426588_v246_n_p_Pallavicini
work_keys_str_mv AT pallavicinic analysisofpersistenceduringintracellularactinbasedtransportmediatedbymolecularmotors
AT despositoma analysisofpersistenceduringintracellularactinbasedtransportmediatedbymolecularmotors
AT leviv analysisofpersistenceduringintracellularactinbasedtransportmediatedbymolecularmotors
AT brunol analysisofpersistenceduringintracellularactinbasedtransportmediatedbymolecularmotors
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