Mechanical strain induces involution-associated events in mammary epithelial cells

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Background: Shortly after weaning, a complex multi-step process that leads to massive epithelial apoptosis is triggered by tissue local factors in the mouse mammary gland. Several reports have demonstrated the relevance of mechanical stress to induce adaptive responses in different cell types. Inter...

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Autores principales:	Quaglino, A., Salierno, M., Pellegrotti, J., Rubinstein, N., Kordon, E.C.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2009
Materias:	leukemia inhibitory factor messenger RNA mitogen activated protein kinase 1 protein c fos protein kinase B silicone STAT3 protein Lif protein, mouse mitogen activated protein kinase 3 Stat3 protein, mouse animal cell animal experiment article artificial membrane Bagg albino mouse breast epithelium cell culture cell stress controlled study device epithelium cell equibiaxial stretching device female image analysis involution mechanical stress microscopy mouse nonhuman protein expression protein phosphorylation theoretical model weaning animal cell line cytology gene expression genetics metabolism phosphorylation pregnancy udder Animals Cell Line Epithelial Cells Female Gene Expression Leukemia Inhibitory Factor Mammary Glands, Animal Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Phosphorylation Pregnancy Proto-Oncogene Proteins c-fos RNA, Messenger STAT3 Transcription Factor Stress, Mechanical
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14712121_v10_n_p_Quaglino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_14712121_v10_n_p_Quaglino
record_format	dspace
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	leukemia inhibitory factor messenger RNA mitogen activated protein kinase 1 protein c fos protein kinase B silicone STAT3 protein leukemia inhibitory factor Lif protein, mouse messenger RNA mitogen activated protein kinase 1 mitogen activated protein kinase 3 protein c fos STAT3 protein Stat3 protein, mouse animal cell animal experiment article artificial membrane Bagg albino mouse breast epithelium cell culture cell stress controlled study device epithelium cell equibiaxial stretching device female image analysis involution mechanical stress microscopy mouse nonhuman protein expression protein phosphorylation theoretical model weaning animal cell line cytology gene expression genetics metabolism phosphorylation pregnancy udder Animals Cell Line Epithelial Cells Female Gene Expression Leukemia Inhibitory Factor Mammary Glands, Animal Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Phosphorylation Pregnancy Proto-Oncogene Proteins c-fos RNA, Messenger STAT3 Transcription Factor Stress, Mechanical
spellingShingle	leukemia inhibitory factor messenger RNA mitogen activated protein kinase 1 protein c fos protein kinase B silicone STAT3 protein leukemia inhibitory factor Lif protein, mouse messenger RNA mitogen activated protein kinase 1 mitogen activated protein kinase 3 protein c fos STAT3 protein Stat3 protein, mouse animal cell animal experiment article artificial membrane Bagg albino mouse breast epithelium cell culture cell stress controlled study device epithelium cell equibiaxial stretching device female image analysis involution mechanical stress microscopy mouse nonhuman protein expression protein phosphorylation theoretical model weaning animal cell line cytology gene expression genetics metabolism phosphorylation pregnancy udder Animals Cell Line Epithelial Cells Female Gene Expression Leukemia Inhibitory Factor Mammary Glands, Animal Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Phosphorylation Pregnancy Proto-Oncogene Proteins c-fos RNA, Messenger STAT3 Transcription Factor Stress, Mechanical Quaglino, A. Salierno, M. Pellegrotti, J. Rubinstein, N. Kordon, E.C. Mechanical strain induces involution-associated events in mammary epithelial cells
topic_facet	leukemia inhibitory factor messenger RNA mitogen activated protein kinase 1 protein c fos protein kinase B silicone STAT3 protein leukemia inhibitory factor Lif protein, mouse messenger RNA mitogen activated protein kinase 1 mitogen activated protein kinase 3 protein c fos STAT3 protein Stat3 protein, mouse animal cell animal experiment article artificial membrane Bagg albino mouse breast epithelium cell culture cell stress controlled study device epithelium cell equibiaxial stretching device female image analysis involution mechanical stress microscopy mouse nonhuman protein expression protein phosphorylation theoretical model weaning animal cell line cytology gene expression genetics metabolism phosphorylation pregnancy udder Animals Cell Line Epithelial Cells Female Gene Expression Leukemia Inhibitory Factor Mammary Glands, Animal Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Phosphorylation Pregnancy Proto-Oncogene Proteins c-fos RNA, Messenger STAT3 Transcription Factor Stress, Mechanical
description	Background: Shortly after weaning, a complex multi-step process that leads to massive epithelial apoptosis is triggered by tissue local factors in the mouse mammary gland. Several reports have demonstrated the relevance of mechanical stress to induce adaptive responses in different cell types. Interestingly, these signaling pathways also participate in mammary gland involution. Then, it has been suggested that cell stretching caused by milk accumulation after weaning might be the first stimulus that initiates the complete remodeling of the mammary gland. However, no previous report has demonstrated the impact of mechanical stress on mammary cell physiology. To address this issue, we have designed a new practical device that allowed us to evaluate the effects of radial stretching on mammary epithelial cells in culture. Results: We have designed and built a new device to analyze the biological consequences of applying mechanical stress to cells cultured on flexible silicone membranes. Subsequently, a geometrical model that predicted the percentage of radial strain applied to the elastic substrate was developed. By microscopic image analysis, the adjustment of these calculations to the actual strain exerted on the attached cells was verified. The studies described herein were all performed in the HC11 non-tumorigenic mammary epithelial cell line, which was originated from a pregnant BALB/c mouse. In these cells, as previously observed in other tissue types, mechanical stress induced ERK1/2 phosphorylation and c-Fos mRNA and protein expression. In addition, we found that mammary cell stretching triggered involution associated cellular events as Leukemia Inhibitory Factor (LIF) expression induction, STAT3 activation and AKT phosphorylation inhibition. Conclusion: Here, we show for the first time, that mechanical strain is able to induce weaning-associated events in cultured mammary epithelial cells. These results were obtained using a new practical and affordable device specifically designed for such a purpose. We believe that our results indicate the relevance of mechanical stress among the early post-lactation events that lead to mammary gland involution. © 2009 Quaglino et al., licensee BioMed Central Ltd.
format	Artículo Artículo publishedVersion
author	Quaglino, A. Salierno, M. Pellegrotti, J. Rubinstein, N. Kordon, E.C.
author_facet	Quaglino, A. Salierno, M. Pellegrotti, J. Rubinstein, N. Kordon, E.C.
author_sort	Quaglino, A.
title	Mechanical strain induces involution-associated events in mammary epithelial cells
title_short	Mechanical strain induces involution-associated events in mammary epithelial cells
title_full	Mechanical strain induces involution-associated events in mammary epithelial cells
title_fullStr	Mechanical strain induces involution-associated events in mammary epithelial cells
title_full_unstemmed	Mechanical strain induces involution-associated events in mammary epithelial cells
title_sort	mechanical strain induces involution-associated events in mammary epithelial cells
publishDate	2009
url	http://hdl.handle.net/20.500.12110/paper_14712121_v10_n_p_Quaglino
work_keys_str_mv	AT quaglinoa mechanicalstraininducesinvolutionassociatedeventsinmammaryepithelialcells AT saliernom mechanicalstraininducesinvolutionassociatedeventsinmammaryepithelialcells AT pellegrottij mechanicalstraininducesinvolutionassociatedeventsinmammaryepithelialcells AT rubinsteinn mechanicalstraininducesinvolutionassociatedeventsinmammaryepithelialcells AT kordonec mechanicalstraininducesinvolutionassociatedeventsinmammaryepithelialcells
_version_	1769810401533362176
spelling	paperaa:paper_14712121_v10_n_p_Quaglino2023-06-12T16:50:23Z Mechanical strain induces involution-associated events in mammary epithelial cells BMC Cell Biol. 2009;10 Quaglino, A. Salierno, M. Pellegrotti, J. Rubinstein, N. Kordon, E.C. leukemia inhibitory factor messenger RNA mitogen activated protein kinase 1 protein c fos protein kinase B silicone STAT3 protein leukemia inhibitory factor Lif protein, mouse messenger RNA mitogen activated protein kinase 1 mitogen activated protein kinase 3 protein c fos STAT3 protein Stat3 protein, mouse animal cell animal experiment article artificial membrane Bagg albino mouse breast epithelium cell culture cell stress controlled study device epithelium cell equibiaxial stretching device female image analysis involution mechanical stress microscopy mouse nonhuman protein expression protein phosphorylation theoretical model weaning animal cell line cytology gene expression genetics metabolism phosphorylation pregnancy udder Animals Cell Line Epithelial Cells Female Gene Expression Leukemia Inhibitory Factor Mammary Glands, Animal Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Phosphorylation Pregnancy Proto-Oncogene Proteins c-fos RNA, Messenger STAT3 Transcription Factor Stress, Mechanical Background: Shortly after weaning, a complex multi-step process that leads to massive epithelial apoptosis is triggered by tissue local factors in the mouse mammary gland. Several reports have demonstrated the relevance of mechanical stress to induce adaptive responses in different cell types. Interestingly, these signaling pathways also participate in mammary gland involution. Then, it has been suggested that cell stretching caused by milk accumulation after weaning might be the first stimulus that initiates the complete remodeling of the mammary gland. However, no previous report has demonstrated the impact of mechanical stress on mammary cell physiology. To address this issue, we have designed a new practical device that allowed us to evaluate the effects of radial stretching on mammary epithelial cells in culture. Results: We have designed and built a new device to analyze the biological consequences of applying mechanical stress to cells cultured on flexible silicone membranes. Subsequently, a geometrical model that predicted the percentage of radial strain applied to the elastic substrate was developed. By microscopic image analysis, the adjustment of these calculations to the actual strain exerted on the attached cells was verified. The studies described herein were all performed in the HC11 non-tumorigenic mammary epithelial cell line, which was originated from a pregnant BALB/c mouse. In these cells, as previously observed in other tissue types, mechanical stress induced ERK1/2 phosphorylation and c-Fos mRNA and protein expression. In addition, we found that mammary cell stretching triggered involution associated cellular events as Leukemia Inhibitory Factor (LIF) expression induction, STAT3 activation and AKT phosphorylation inhibition. Conclusion: Here, we show for the first time, that mechanical strain is able to induce weaning-associated events in cultured mammary epithelial cells. These results were obtained using a new practical and affordable device specifically designed for such a purpose. We believe that our results indicate the relevance of mechanical stress among the early post-lactation events that lead to mammary gland involution. © 2009 Quaglino et al., licensee BioMed Central Ltd. Fil:Quaglino, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Salierno, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rubinstein, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kordon, E.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14712121_v10_n_p_Quaglino

Mechanical strain induces involution-associated events in mammary epithelial cells

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