A positive correlation between bacterial autoaggregation and biofilm formation in native Sinorhizobium meliloti isolates from Argentina

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Sinorhizobium meliloti is a symbiotic nitrogen-fixing bacterium that elicits nodule formation on roots of alfalfa plants. S. meliloti produces two exopolysaccharides (EPSs), termed EPS I and EPS II, that are both able to promote symbiosis. EPS I and EPS II are secreted in two major fractions that re...

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Autores principales:	Sorroche, F.G., Spesia, M.B., Zorreguieta, Á., Giordano, W.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2012
Materias:	16S rRNA gene Adhesive force Argentina Biofilm formation Cell-cell interaction Complementation Exopolysaccharides Extracellular Insertion sequences Nitrogen fixing bacteria PCR analysis Positive correlations Reference strains Root nodules Sinorhizobium meliloti Surface attachment Synthetic pathways Bacteria Genes Nitrogen fixation RNA Biofilms bacterial DNA bacterial polysaccharide ribosome DNA RNA 16S aggregation alfalfa bacterium biofilm genetic analysis mutation nitrogen fixation nodulation polymerase chain reaction polymerization symbiont symbiosis article bacterium adherence chemistry classification cluster analysis DNA sequence genetics growth, development and aging isolation and purification metabolism microbiology molecular genetics nucleotide sequence phylogeny physiology plant root Bacterial Adhesion Cluster Analysis DNA, Bacterial DNA, Ribosomal Medicago sativa Molecular Sequence Data Phylogeny Plant Roots Polysaccharides, Bacterial RNA, Ribosomal, 16S Sequence Analysis, DNA Bacteria (microorganisms)
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00992240_v78_n12_p4092_Sorroche
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_00992240_v78_n12_p4092_Sorroche
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spelling	paperaa:paper_00992240_v78_n12_p4092_Sorroche2023-06-12T16:46:24Z A positive correlation between bacterial autoaggregation and biofilm formation in native Sinorhizobium meliloti isolates from Argentina Appl. Environ. Microbiol. 2012;78(12):4092-4101 Sorroche, F.G. Spesia, M.B. Zorreguieta, Á. Giordano, W. 16S rRNA gene Adhesive force Argentina Biofilm formation Cell-cell interaction Complementation Exopolysaccharides Extracellular Insertion sequences Nitrogen fixing bacteria PCR analysis Positive correlations Reference strains Root nodules Sinorhizobium meliloti Surface attachment Synthetic pathways Bacteria Genes Nitrogen fixation RNA Biofilms bacterial DNA bacterial polysaccharide ribosome DNA RNA 16S aggregation alfalfa bacterium biofilm genetic analysis mutation nitrogen fixation nodulation polymerase chain reaction polymerization symbiont symbiosis alfalfa Argentina article bacterium adherence biofilm chemistry classification cluster analysis DNA sequence genetics growth, development and aging isolation and purification metabolism microbiology molecular genetics nucleotide sequence phylogeny physiology plant root Sinorhizobium meliloti Argentina Bacterial Adhesion Biofilms Cluster Analysis DNA, Bacterial DNA, Ribosomal Medicago sativa Molecular Sequence Data Phylogeny Plant Roots Polysaccharides, Bacterial RNA, Ribosomal, 16S Sequence Analysis, DNA Sinorhizobium meliloti Argentina Bacteria (microorganisms) Medicago sativa Sinorhizobium meliloti Sinorhizobium meliloti is a symbiotic nitrogen-fixing bacterium that elicits nodule formation on roots of alfalfa plants. S. meliloti produces two exopolysaccharides (EPSs), termed EPS I and EPS II, that are both able to promote symbiosis. EPS I and EPS II are secreted in two major fractions that reflect differing degrees of subunit polymerization, designated high- and low-molecularweight fractions. We reported previously that EPSs are crucial for autoaggregation and biofilm formation in S. meliloti reference strains and isogenic mutants. However, the previous observations were obtained by use of "domesticated" laboratory strains, with mutations resulting from successive passages under unnatural conditions, as has been documented for reference strain Rm1021. In the present study, we analyzed the autoaggregation and biofilm formation abilities of native S. meliloti strains isolated from root nodules of alfalfa plants grown in four regions of Argentina. 16S rRNA gene analysis of all the native isolates revealed a high degree of identity with reference S. meliloti strains. PCR analysis of the expR gene of all the isolates showed that, as in the case of reference strain Rm8530, this gene is not interrupted by an insertion sequence (IS) element. A positive correlation was found between autoaggregation and biofilm formation abilities in these rhizobia, indicating that both processes depend on the same physical adhesive forces. Extracellular complementation experiments using mutants of the native strains showed that autoaggregation was dependent on EPS II production. Our results indicate that a functional EPS II synthetic pathway and its proper regulation are essential for cell-cell interactions and surface attachment of S. meliloti. © 2012, American Society for Microbiology. Fil:Zorreguieta, Á. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00992240_v78_n12_p4092_Sorroche
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	16S rRNA gene Adhesive force Argentina Biofilm formation Cell-cell interaction Complementation Exopolysaccharides Extracellular Insertion sequences Nitrogen fixing bacteria PCR analysis Positive correlations Reference strains Root nodules Sinorhizobium meliloti Surface attachment Synthetic pathways Bacteria Genes Nitrogen fixation RNA Biofilms bacterial DNA bacterial polysaccharide ribosome DNA RNA 16S aggregation alfalfa bacterium biofilm genetic analysis mutation nitrogen fixation nodulation polymerase chain reaction polymerization symbiont symbiosis alfalfa Argentina article bacterium adherence biofilm chemistry classification cluster analysis DNA sequence genetics growth, development and aging isolation and purification metabolism microbiology molecular genetics nucleotide sequence phylogeny physiology plant root Sinorhizobium meliloti Argentina Bacterial Adhesion Biofilms Cluster Analysis DNA, Bacterial DNA, Ribosomal Medicago sativa Molecular Sequence Data Phylogeny Plant Roots Polysaccharides, Bacterial RNA, Ribosomal, 16S Sequence Analysis, DNA Sinorhizobium meliloti Argentina Bacteria (microorganisms) Medicago sativa Sinorhizobium meliloti
spellingShingle	16S rRNA gene Adhesive force Argentina Biofilm formation Cell-cell interaction Complementation Exopolysaccharides Extracellular Insertion sequences Nitrogen fixing bacteria PCR analysis Positive correlations Reference strains Root nodules Sinorhizobium meliloti Surface attachment Synthetic pathways Bacteria Genes Nitrogen fixation RNA Biofilms bacterial DNA bacterial polysaccharide ribosome DNA RNA 16S aggregation alfalfa bacterium biofilm genetic analysis mutation nitrogen fixation nodulation polymerase chain reaction polymerization symbiont symbiosis alfalfa Argentina article bacterium adherence biofilm chemistry classification cluster analysis DNA sequence genetics growth, development and aging isolation and purification metabolism microbiology molecular genetics nucleotide sequence phylogeny physiology plant root Sinorhizobium meliloti Argentina Bacterial Adhesion Biofilms Cluster Analysis DNA, Bacterial DNA, Ribosomal Medicago sativa Molecular Sequence Data Phylogeny Plant Roots Polysaccharides, Bacterial RNA, Ribosomal, 16S Sequence Analysis, DNA Sinorhizobium meliloti Argentina Bacteria (microorganisms) Medicago sativa Sinorhizobium meliloti Sorroche, F.G. Spesia, M.B. Zorreguieta, Á. Giordano, W. A positive correlation between bacterial autoaggregation and biofilm formation in native Sinorhizobium meliloti isolates from Argentina
topic_facet	16S rRNA gene Adhesive force Argentina Biofilm formation Cell-cell interaction Complementation Exopolysaccharides Extracellular Insertion sequences Nitrogen fixing bacteria PCR analysis Positive correlations Reference strains Root nodules Sinorhizobium meliloti Surface attachment Synthetic pathways Bacteria Genes Nitrogen fixation RNA Biofilms bacterial DNA bacterial polysaccharide ribosome DNA RNA 16S aggregation alfalfa bacterium biofilm genetic analysis mutation nitrogen fixation nodulation polymerase chain reaction polymerization symbiont symbiosis alfalfa Argentina article bacterium adherence biofilm chemistry classification cluster analysis DNA sequence genetics growth, development and aging isolation and purification metabolism microbiology molecular genetics nucleotide sequence phylogeny physiology plant root Sinorhizobium meliloti Argentina Bacterial Adhesion Biofilms Cluster Analysis DNA, Bacterial DNA, Ribosomal Medicago sativa Molecular Sequence Data Phylogeny Plant Roots Polysaccharides, Bacterial RNA, Ribosomal, 16S Sequence Analysis, DNA Sinorhizobium meliloti Argentina Bacteria (microorganisms) Medicago sativa Sinorhizobium meliloti
description	Sinorhizobium meliloti is a symbiotic nitrogen-fixing bacterium that elicits nodule formation on roots of alfalfa plants. S. meliloti produces two exopolysaccharides (EPSs), termed EPS I and EPS II, that are both able to promote symbiosis. EPS I and EPS II are secreted in two major fractions that reflect differing degrees of subunit polymerization, designated high- and low-molecularweight fractions. We reported previously that EPSs are crucial for autoaggregation and biofilm formation in S. meliloti reference strains and isogenic mutants. However, the previous observations were obtained by use of "domesticated" laboratory strains, with mutations resulting from successive passages under unnatural conditions, as has been documented for reference strain Rm1021. In the present study, we analyzed the autoaggregation and biofilm formation abilities of native S. meliloti strains isolated from root nodules of alfalfa plants grown in four regions of Argentina. 16S rRNA gene analysis of all the native isolates revealed a high degree of identity with reference S. meliloti strains. PCR analysis of the expR gene of all the isolates showed that, as in the case of reference strain Rm8530, this gene is not interrupted by an insertion sequence (IS) element. A positive correlation was found between autoaggregation and biofilm formation abilities in these rhizobia, indicating that both processes depend on the same physical adhesive forces. Extracellular complementation experiments using mutants of the native strains showed that autoaggregation was dependent on EPS II production. Our results indicate that a functional EPS II synthetic pathway and its proper regulation are essential for cell-cell interactions and surface attachment of S. meliloti. © 2012, American Society for Microbiology.
format	Artículo Artículo publishedVersion
author	Sorroche, F.G. Spesia, M.B. Zorreguieta, Á. Giordano, W.
author_facet	Sorroche, F.G. Spesia, M.B. Zorreguieta, Á. Giordano, W.
author_sort	Sorroche, F.G.
title	A positive correlation between bacterial autoaggregation and biofilm formation in native Sinorhizobium meliloti isolates from Argentina
title_short	A positive correlation between bacterial autoaggregation and biofilm formation in native Sinorhizobium meliloti isolates from Argentina
title_full	A positive correlation between bacterial autoaggregation and biofilm formation in native Sinorhizobium meliloti isolates from Argentina
title_fullStr	A positive correlation between bacterial autoaggregation and biofilm formation in native Sinorhizobium meliloti isolates from Argentina
title_full_unstemmed	A positive correlation between bacterial autoaggregation and biofilm formation in native Sinorhizobium meliloti isolates from Argentina
title_sort	positive correlation between bacterial autoaggregation and biofilm formation in native sinorhizobium meliloti isolates from argentina
publishDate	2012
url	http://hdl.handle.net/20.500.12110/paper_00992240_v78_n12_p4092_Sorroche
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A positive correlation between bacterial autoaggregation and biofilm formation in native Sinorhizobium meliloti isolates from Argentina

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