Trypanosoma cruzi surface mucins with exposed variant epitopes

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The protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and surviv...

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Autores principales:	Pollevick, G.D., Di Noia, J.M., Salto, M.L., Lima, C., Leguizamón, M.S., De Lederkremer, R.M., Frasch, A.C.C.
Formato:	Artículo publishedVersion
Lenguaje:	Inglés
Publicado:	2000
Materias:	epitope gene product glycosylphosphatidylinositol monosaccharide mucin sialic acid amino terminal sequence animal cell article cell interaction cell membrane Chagas disease gene expression gene sequence genetic transfection mammal multigene family nonhuman polyacrylamide gel electrophoresis priority journal protein expression tandem repeat Trypanosoma cruzi vertebrate Amino Acid Sequence Animals Antigens, Protozoan Base Sequence Carbohydrate Sequence Chagas Disease Epitopes Glycosylation Glycosylphosphatidylinositols Mice Molecular Sequence Data Mucins Oligosaccharides Recombinant Proteins Transfection Variation (Genetics) Animalia Insecta Mammalia Protozoa Trypanosoma Vertebrata
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00219258_v275_n36_p27671_Pollevick
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paperaa:paper_00219258_v275_n36_p27671_Pollevick
record_format	dspace
spelling	paperaa:paper_00219258_v275_n36_p27671_Pollevick2023-06-12T16:42:48Z Trypanosoma cruzi surface mucins with exposed variant epitopes J. Biol. Chem. 2000;275(36):27671-27680 Pollevick, G.D. Di Noia, J.M. Salto, M.L. Lima, C. Leguizamón, M.S. De Lederkremer, R.M. Frasch, A.C.C. epitope gene product glycosylphosphatidylinositol monosaccharide mucin sialic acid amino terminal sequence animal cell article cell interaction cell membrane Chagas disease gene expression gene sequence genetic transfection mammal multigene family nonhuman polyacrylamide gel electrophoresis priority journal protein expression tandem repeat Trypanosoma cruzi vertebrate Amino Acid Sequence Animals Antigens, Protozoan Base Sequence Carbohydrate Sequence Chagas Disease Epitopes Glycosylation Glycosylphosphatidylinositols Mice Molecular Sequence Data Mucins Oligosaccharides Recombinant Proteins Transfection Trypanosoma cruzi Variation (Genetics) Animalia Insecta Mammalia Protozoa Trypanosoma Trypanosoma cruzi Vertebrata The protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and survive in the mammalian host. A large mucin-like gene family named TcMUC containing about 500 members has been identified previously in T. cruzi. TcMUC can be divided into two subfamilies according to the presence or absence of tandem repeats in the central region of the genes. In this work, T. cruzi parasites were transfected with one tagged member of each subfamily. Only the product from the gene with repeats was highly O-glycosylated in vivo. The O-linked oligosaccharides consisted mainly of β-D-Galp(1→4)-GlcNAc and β-D-Galp(1→4)[β-D-Galp(1→6)]-D-GlcNAc. The same glycosyl moieties were found in endogenous mucins. The mature product was anchored by glycosylphosphatidylinositol to the plasma membrane and exposed to the medium. Sera from infected mice recognized the recombinant product of one repeats-containing gene thus showing that they are expressed during the infection. TcMUC genes encode a hypervariable region at the N terminus. We now show that the hypervariable region is indeed present in the exposed mature N termini of the mucins because sera from infected hosts recognized peptides having sequences from this region. The results are discussed in comparison with the mucins from the insect stages of the parasite (Di Noia, J. M., D'Orso, I., Sanchez, D. O., and Frasch, A. C. C. (2000) J. Biol. Chem. 275, 10218-10227) which do not have variable regions. Fil:Pollevick, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Noia, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Salto, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leguizamón, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219258_v275_n36_p27671_Pollevick
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	Inglés
orig_language_str_mv	eng
topic	epitope gene product glycosylphosphatidylinositol monosaccharide mucin sialic acid amino terminal sequence animal cell article cell interaction cell membrane Chagas disease gene expression gene sequence genetic transfection mammal multigene family nonhuman polyacrylamide gel electrophoresis priority journal protein expression tandem repeat Trypanosoma cruzi vertebrate Amino Acid Sequence Animals Antigens, Protozoan Base Sequence Carbohydrate Sequence Chagas Disease Epitopes Glycosylation Glycosylphosphatidylinositols Mice Molecular Sequence Data Mucins Oligosaccharides Recombinant Proteins Transfection Trypanosoma cruzi Variation (Genetics) Animalia Insecta Mammalia Protozoa Trypanosoma Trypanosoma cruzi Vertebrata
spellingShingle	epitope gene product glycosylphosphatidylinositol monosaccharide mucin sialic acid amino terminal sequence animal cell article cell interaction cell membrane Chagas disease gene expression gene sequence genetic transfection mammal multigene family nonhuman polyacrylamide gel electrophoresis priority journal protein expression tandem repeat Trypanosoma cruzi vertebrate Amino Acid Sequence Animals Antigens, Protozoan Base Sequence Carbohydrate Sequence Chagas Disease Epitopes Glycosylation Glycosylphosphatidylinositols Mice Molecular Sequence Data Mucins Oligosaccharides Recombinant Proteins Transfection Trypanosoma cruzi Variation (Genetics) Animalia Insecta Mammalia Protozoa Trypanosoma Trypanosoma cruzi Vertebrata Pollevick, G.D. Di Noia, J.M. Salto, M.L. Lima, C. Leguizamón, M.S. De Lederkremer, R.M. Frasch, A.C.C. Trypanosoma cruzi surface mucins with exposed variant epitopes
topic_facet	epitope gene product glycosylphosphatidylinositol monosaccharide mucin sialic acid amino terminal sequence animal cell article cell interaction cell membrane Chagas disease gene expression gene sequence genetic transfection mammal multigene family nonhuman polyacrylamide gel electrophoresis priority journal protein expression tandem repeat Trypanosoma cruzi vertebrate Amino Acid Sequence Animals Antigens, Protozoan Base Sequence Carbohydrate Sequence Chagas Disease Epitopes Glycosylation Glycosylphosphatidylinositols Mice Molecular Sequence Data Mucins Oligosaccharides Recombinant Proteins Transfection Trypanosoma cruzi Variation (Genetics) Animalia Insecta Mammalia Protozoa Trypanosoma Trypanosoma cruzi Vertebrata
description	The protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and survive in the mammalian host. A large mucin-like gene family named TcMUC containing about 500 members has been identified previously in T. cruzi. TcMUC can be divided into two subfamilies according to the presence or absence of tandem repeats in the central region of the genes. In this work, T. cruzi parasites were transfected with one tagged member of each subfamily. Only the product from the gene with repeats was highly O-glycosylated in vivo. The O-linked oligosaccharides consisted mainly of β-D-Galp(1→4)-GlcNAc and β-D-Galp(1→4)[β-D-Galp(1→6)]-D-GlcNAc. The same glycosyl moieties were found in endogenous mucins. The mature product was anchored by glycosylphosphatidylinositol to the plasma membrane and exposed to the medium. Sera from infected mice recognized the recombinant product of one repeats-containing gene thus showing that they are expressed during the infection. TcMUC genes encode a hypervariable region at the N terminus. We now show that the hypervariable region is indeed present in the exposed mature N termini of the mucins because sera from infected hosts recognized peptides having sequences from this region. The results are discussed in comparison with the mucins from the insect stages of the parasite (Di Noia, J. M., D'Orso, I., Sanchez, D. O., and Frasch, A. C. C. (2000) J. Biol. Chem. 275, 10218-10227) which do not have variable regions.
format	Artículo Artículo publishedVersion
author	Pollevick, G.D. Di Noia, J.M. Salto, M.L. Lima, C. Leguizamón, M.S. De Lederkremer, R.M. Frasch, A.C.C.
author_facet	Pollevick, G.D. Di Noia, J.M. Salto, M.L. Lima, C. Leguizamón, M.S. De Lederkremer, R.M. Frasch, A.C.C.
author_sort	Pollevick, G.D.
title	Trypanosoma cruzi surface mucins with exposed variant epitopes
title_short	Trypanosoma cruzi surface mucins with exposed variant epitopes
title_full	Trypanosoma cruzi surface mucins with exposed variant epitopes
title_fullStr	Trypanosoma cruzi surface mucins with exposed variant epitopes
title_full_unstemmed	Trypanosoma cruzi surface mucins with exposed variant epitopes
title_sort	trypanosoma cruzi surface mucins with exposed variant epitopes
publishDate	2000
url	http://hdl.handle.net/20.500.12110/paper_00219258_v275_n36_p27671_Pollevick
work_keys_str_mv	AT pollevickgd trypanosomacruzisurfacemucinswithexposedvariantepitopes AT dinoiajm trypanosomacruzisurfacemucinswithexposedvariantepitopes AT saltoml trypanosomacruzisurfacemucinswithexposedvariantepitopes AT limac trypanosomacruzisurfacemucinswithexposedvariantepitopes AT leguizamonms trypanosomacruzisurfacemucinswithexposedvariantepitopes AT delederkremerrm trypanosomacruzisurfacemucinswithexposedvariantepitopes AT fraschacc trypanosomacruzisurfacemucinswithexposedvariantepitopes
_version_	1769810316476022784

Trypanosoma cruzi surface mucins with exposed variant epitopes

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