A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals

IFN-γ release assays (IGRAs) are better indicators of Mycobacterium tuberculosis infection than the tuberculin skin test (TST) in Bacillus Calmette-Guérin (BCG)-vaccinated populations. However, IGRAs do not discriminate active and latent infections (LTBI) and no gold standard for LTBI diagnosis is a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Peña, Delfina, Amiano, Nicolás Oscar, Pasquinelli, Virginia, Gherardi, María Magdalena, García, Verónica Edith
Publicado:	2015
Materias:	Active tuberculosis Antigens Diagnosis IFN-gamma Latent tuberculosis epitope gamma interferon bacterial antigen BCG vaccine IFNG protein, human adult Article BCG vaccination controlled study enzyme linked immunosorbent assay female flow cytometry human latent virus infection major clinical study male Mycobacterium tuberculosis nonhuman peripheral blood mononuclear cell priority journal sequence analysis tuberculin test tuberculosis immunology middle aged mononuclear cell Mycobacterium bovis Adult Antigens, Bacterial Female Humans Interferon-gamma Leukocytes, Mononuclear Male Middle Aged Tuberculosis Vaccines
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23523964_v2_n8_p884_Pena http://hdl.handle.net/20.500.12110/paper_23523964_v2_n8_p884_Pena
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_23523964_v2_n8_p884_Pena
record_format	dspace
spelling	paper:paper_23523964_v2_n8_p884_Pena2023-06-08T16:35:43Z A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals Peña, Delfina Amiano, Nicolás Oscar Pasquinelli, Virginia Gherardi, María Magdalena García, Verónica Edith Active tuberculosis Antigens Diagnosis IFN-gamma Latent tuberculosis epitope gamma interferon bacterial antigen BCG vaccine gamma interferon IFNG protein, human adult Article BCG vaccination controlled study enzyme linked immunosorbent assay female flow cytometry human latent virus infection major clinical study male Mycobacterium tuberculosis nonhuman peripheral blood mononuclear cell priority journal sequence analysis tuberculin test tuberculosis immunology middle aged mononuclear cell Mycobacterium bovis Adult Antigens, Bacterial Female Humans Interferon-gamma Leukocytes, Mononuclear Male Middle Aged Mycobacterium bovis Mycobacterium tuberculosis Tuberculosis Vaccines IFN-γ release assays (IGRAs) are better indicators of Mycobacterium tuberculosis infection than the tuberculin skin test (TST) in Bacillus Calmette-Guérin (BCG)-vaccinated populations. However, IGRAs do not discriminate active and latent infections (LTBI) and no gold standard for LTBI diagnosis is available. Thus, since improved tests to diagnose M. tuberculosis infection are required, we assessed the efficacy of several M. tuberculosis latency antigens. BCG-vaccinated healthy donors (HD) and tuberculosis (TB) patients were recruited. QuantiFERON-TB Gold In-Tube, TST and clinical data were used to differentiate LTBI. IFN-γ production against CFP-10, ESAT-6, Rv2624c, Rv2626c and Rv2628 antigens was tested in peripheral blood mononuclear cells. LTBI subjects secreted significantly higher IFN-γ levels against Rv2626c than HD. Additionally, Rv2626c peptide pools to which only LTBI responded were identified, and their cumulative IFN-γ response improved LTBI discrimination. Interestingly, whole blood stimulation with Rv2626c allowed the discrimination between active and latent infections, since TB patients did not secrete IFN-γ against Rv2626c, in contrast to CFP-10. +. ESAT-6 stimulation that induced IFN-γ response from both LTBI and TB patients. ROC analysis confirmed that Rv2626c discriminated LTBI from HD and TB patients. Therefore, since only LTBI recognizes specific epitopes from Rv2626c, this antigen could improve LTBI diagnosis, even in BCG-vaccinated people. © 2015 The Authors. Fil:Peña, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Amiano, N.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pasquinelli, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gherardi, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:García, V.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23523964_v2_n8_p884_Pena http://hdl.handle.net/20.500.12110/paper_23523964_v2_n8_p884_Pena
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Active tuberculosis Antigens Diagnosis IFN-gamma Latent tuberculosis epitope gamma interferon bacterial antigen BCG vaccine gamma interferon IFNG protein, human adult Article BCG vaccination controlled study enzyme linked immunosorbent assay female flow cytometry human latent virus infection major clinical study male Mycobacterium tuberculosis nonhuman peripheral blood mononuclear cell priority journal sequence analysis tuberculin test tuberculosis immunology middle aged mononuclear cell Mycobacterium bovis Adult Antigens, Bacterial Female Humans Interferon-gamma Leukocytes, Mononuclear Male Middle Aged Mycobacterium bovis Mycobacterium tuberculosis Tuberculosis Vaccines
spellingShingle	Active tuberculosis Antigens Diagnosis IFN-gamma Latent tuberculosis epitope gamma interferon bacterial antigen BCG vaccine gamma interferon IFNG protein, human adult Article BCG vaccination controlled study enzyme linked immunosorbent assay female flow cytometry human latent virus infection major clinical study male Mycobacterium tuberculosis nonhuman peripheral blood mononuclear cell priority journal sequence analysis tuberculin test tuberculosis immunology middle aged mononuclear cell Mycobacterium bovis Adult Antigens, Bacterial Female Humans Interferon-gamma Leukocytes, Mononuclear Male Middle Aged Mycobacterium bovis Mycobacterium tuberculosis Tuberculosis Vaccines Peña, Delfina Amiano, Nicolás Oscar Pasquinelli, Virginia Gherardi, María Magdalena García, Verónica Edith A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals
topic_facet	Active tuberculosis Antigens Diagnosis IFN-gamma Latent tuberculosis epitope gamma interferon bacterial antigen BCG vaccine gamma interferon IFNG protein, human adult Article BCG vaccination controlled study enzyme linked immunosorbent assay female flow cytometry human latent virus infection major clinical study male Mycobacterium tuberculosis nonhuman peripheral blood mononuclear cell priority journal sequence analysis tuberculin test tuberculosis immunology middle aged mononuclear cell Mycobacterium bovis Adult Antigens, Bacterial Female Humans Interferon-gamma Leukocytes, Mononuclear Male Middle Aged Mycobacterium bovis Mycobacterium tuberculosis Tuberculosis Vaccines
description	IFN-γ release assays (IGRAs) are better indicators of Mycobacterium tuberculosis infection than the tuberculin skin test (TST) in Bacillus Calmette-Guérin (BCG)-vaccinated populations. However, IGRAs do not discriminate active and latent infections (LTBI) and no gold standard for LTBI diagnosis is available. Thus, since improved tests to diagnose M. tuberculosis infection are required, we assessed the efficacy of several M. tuberculosis latency antigens. BCG-vaccinated healthy donors (HD) and tuberculosis (TB) patients were recruited. QuantiFERON-TB Gold In-Tube, TST and clinical data were used to differentiate LTBI. IFN-γ production against CFP-10, ESAT-6, Rv2624c, Rv2626c and Rv2628 antigens was tested in peripheral blood mononuclear cells. LTBI subjects secreted significantly higher IFN-γ levels against Rv2626c than HD. Additionally, Rv2626c peptide pools to which only LTBI responded were identified, and their cumulative IFN-γ response improved LTBI discrimination. Interestingly, whole blood stimulation with Rv2626c allowed the discrimination between active and latent infections, since TB patients did not secrete IFN-γ against Rv2626c, in contrast to CFP-10. +. ESAT-6 stimulation that induced IFN-γ response from both LTBI and TB patients. ROC analysis confirmed that Rv2626c discriminated LTBI from HD and TB patients. Therefore, since only LTBI recognizes specific epitopes from Rv2626c, this antigen could improve LTBI diagnosis, even in BCG-vaccinated people. © 2015 The Authors.
author	Peña, Delfina Amiano, Nicolás Oscar Pasquinelli, Virginia Gherardi, María Magdalena García, Verónica Edith
author_facet	Peña, Delfina Amiano, Nicolás Oscar Pasquinelli, Virginia Gherardi, María Magdalena García, Verónica Edith
author_sort	Peña, Delfina
title	A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals
title_short	A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals
title_full	A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals
title_fullStr	A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals
title_full_unstemmed	A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals
title_sort	mycobacterium tuberculosis dormancy antigen differentiates latently infected bacillus calmette-guérin-vaccinated individuals
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23523964_v2_n8_p884_Pena http://hdl.handle.net/20.500.12110/paper_23523964_v2_n8_p884_Pena
work_keys_str_mv	AT penadelfina amycobacteriumtuberculosisdormancyantigendifferentiateslatentlyinfectedbacilluscalmetteguerinvaccinatedindividuals AT amianonicolasoscar amycobacteriumtuberculosisdormancyantigendifferentiateslatentlyinfectedbacilluscalmetteguerinvaccinatedindividuals AT pasquinellivirginia amycobacteriumtuberculosisdormancyantigendifferentiateslatentlyinfectedbacilluscalmetteguerinvaccinatedindividuals AT gherardimariamagdalena amycobacteriumtuberculosisdormancyantigendifferentiateslatentlyinfectedbacilluscalmetteguerinvaccinatedindividuals AT garciaveronicaedith amycobacteriumtuberculosisdormancyantigendifferentiateslatentlyinfectedbacilluscalmetteguerinvaccinatedindividuals AT penadelfina mycobacteriumtuberculosisdormancyantigendifferentiateslatentlyinfectedbacilluscalmetteguerinvaccinatedindividuals AT amianonicolasoscar mycobacteriumtuberculosisdormancyantigendifferentiateslatentlyinfectedbacilluscalmetteguerinvaccinatedindividuals AT pasquinellivirginia mycobacteriumtuberculosisdormancyantigendifferentiateslatentlyinfectedbacilluscalmetteguerinvaccinatedindividuals AT gherardimariamagdalena mycobacteriumtuberculosisdormancyantigendifferentiateslatentlyinfectedbacilluscalmetteguerinvaccinatedindividuals AT garciaveronicaedith mycobacteriumtuberculosisdormancyantigendifferentiateslatentlyinfectedbacilluscalmetteguerinvaccinatedindividuals
_version_	1768543442350112768

A Mycobacterium tuberculosis Dormancy Antigen Differentiates Latently Infected Bacillus Calmette-Guérin-vaccinated Individuals

Ejemplares similares