Co-imaging extrinsic, intrinsic and effector caspase activity by fluorescence anisotropy microscopy

In order to overcome intercellular variability and thereby effectively assess signal propagation in biological networks it is imperative to simultaneously quantify multiple biological observables in single living cells. While fluorescent biosensors have been the tool of choice to monitor the dynamic...

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Detalles Bibliográficos
Publicado:	2018
Materias:	Anisotropy FRET biosensor Apoptotic network Caspase activity Co-monitoring Imaging Polarization microscopy caspase 3 caspase 8 caspase 9 effector caspase anisotropy anisotropy forster resonant energy transfer based biosensor apoptosis Article controlled study correlation analysis enzyme activation enzyme activity fluorescence anisotropy microscopy human human cell microscopy molecular model priority journal simulation fluorescence microscopy fluorescence polarization fluorescence resonance energy transfer genetic procedures HeLa cell line metabolism procedures signal transduction Apoptosis Biosensing Techniques Caspases, Effector Enzyme Activation Fluorescence Polarization Fluorescence Resonance Energy Transfer HeLa Cells Humans Microscopy, Fluorescence Signal Transduction
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_22132317_v19_n_p210_Corbat http://hdl.handle.net/20.500.12110/paper_22132317_v19_n_p210_Corbat
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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