Internalization of locked nucleic acids/DNA hybrid oligomers into escherichia coli
Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia c...
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2012
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21647860_v1_n5_p260_Traglia http://hdl.handle.net/20.500.12110/paper_21647860_v1_n5_p260_Traglia |
Aporte de: |
Sumario: | Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cytoplasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a sta- tionary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm.© Mary Ann Liebert, Inc. |
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