Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides
Protein kinase A (PKA) activity is involved in dimorphism of the basal fungal lineage Mucor. From the recently sequenced genome of Mucor circinelloides we could predict ten catalytic subunits of PKA. From sequence alignment and structural prediction we conclude that the catalytic core of the isoform...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18786146_v120_n12_p1493_FernandezNunez http://hdl.handle.net/20.500.12110/paper_18786146_v120_n12_p1493_FernandezNunez |
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paper:paper_18786146_v120_n12_p1493_FernandezNunez2023-06-08T16:30:18Z Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides Ocampo, Josefina Rossi, Silvia Graciela Moreno, Silvia cAMP Differentiation Orthologs Paralogs Pseudokinase Zygomycetes biochemistry catalysis chemical property cladistics dimorphism enzyme activity fungus gene expression genome growth protein Fungi Mucor Mucor circinelloides Zygomycetes cyclic AMP dependent protein kinase isoprotein messenger RNA aerobic metabolism anaerobic growth enzyme active site enzymology gene expression profiling genetics growth, development and aging Mucor sequence homology Aerobiosis Anaerobiosis Catalytic Domain Cyclic AMP-Dependent Protein Kinases Gene Expression Profiling Mucor Protein Isoforms RNA, Messenger Sequence Homology Protein kinase A (PKA) activity is involved in dimorphism of the basal fungal lineage Mucor. From the recently sequenced genome of Mucor circinelloides we could predict ten catalytic subunits of PKA. From sequence alignment and structural prediction we conclude that the catalytic core of the isoforms is conserved, and the difference between them resides in their amino termini. This high number of isoforms is maintained in the subdivision Mucoromycotina. Each paralogue, when compared to the ones form other fungi is more homologous to one of its orthologs than to its paralogs. All of these fungal isoforms cannot be included in the class I or II in which fungal protein kinases have been classified. mRNA levels for each isoform were measured during aerobic and anaerobic growth. The expression of each isoform is differential and associated to a particular growth stage. We reanalyzed the sequence of PKAC (GI 20218944), the only cloned sequence available until now for a catalytic subunit of M. circinelloides. PKAC cannot be classified as a PKA because of its difference in the conserved C-tail; it shares with PKB a conserved C2 domain in the N-terminus. No catalytic activity could be measured for this protein nor predicted bioinformatically. It can thus be classified as a pseudokinase. Its importance can not be underestimated since it is expressed at the mRNA level in different stages of growth, and its deletion is lethal. © 2016 British Mycological Society Fil:Ocampo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossi, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18786146_v120_n12_p1493_FernandezNunez http://hdl.handle.net/20.500.12110/paper_18786146_v120_n12_p1493_FernandezNunez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cAMP Differentiation Orthologs Paralogs Pseudokinase Zygomycetes biochemistry catalysis chemical property cladistics dimorphism enzyme activity fungus gene expression genome growth protein Fungi Mucor Mucor circinelloides Zygomycetes cyclic AMP dependent protein kinase isoprotein messenger RNA aerobic metabolism anaerobic growth enzyme active site enzymology gene expression profiling genetics growth, development and aging Mucor sequence homology Aerobiosis Anaerobiosis Catalytic Domain Cyclic AMP-Dependent Protein Kinases Gene Expression Profiling Mucor Protein Isoforms RNA, Messenger Sequence Homology |
| spellingShingle |
cAMP Differentiation Orthologs Paralogs Pseudokinase Zygomycetes biochemistry catalysis chemical property cladistics dimorphism enzyme activity fungus gene expression genome growth protein Fungi Mucor Mucor circinelloides Zygomycetes cyclic AMP dependent protein kinase isoprotein messenger RNA aerobic metabolism anaerobic growth enzyme active site enzymology gene expression profiling genetics growth, development and aging Mucor sequence homology Aerobiosis Anaerobiosis Catalytic Domain Cyclic AMP-Dependent Protein Kinases Gene Expression Profiling Mucor Protein Isoforms RNA, Messenger Sequence Homology Ocampo, Josefina Rossi, Silvia Graciela Moreno, Silvia Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides |
| topic_facet |
cAMP Differentiation Orthologs Paralogs Pseudokinase Zygomycetes biochemistry catalysis chemical property cladistics dimorphism enzyme activity fungus gene expression genome growth protein Fungi Mucor Mucor circinelloides Zygomycetes cyclic AMP dependent protein kinase isoprotein messenger RNA aerobic metabolism anaerobic growth enzyme active site enzymology gene expression profiling genetics growth, development and aging Mucor sequence homology Aerobiosis Anaerobiosis Catalytic Domain Cyclic AMP-Dependent Protein Kinases Gene Expression Profiling Mucor Protein Isoforms RNA, Messenger Sequence Homology |
| description |
Protein kinase A (PKA) activity is involved in dimorphism of the basal fungal lineage Mucor. From the recently sequenced genome of Mucor circinelloides we could predict ten catalytic subunits of PKA. From sequence alignment and structural prediction we conclude that the catalytic core of the isoforms is conserved, and the difference between them resides in their amino termini. This high number of isoforms is maintained in the subdivision Mucoromycotina. Each paralogue, when compared to the ones form other fungi is more homologous to one of its orthologs than to its paralogs. All of these fungal isoforms cannot be included in the class I or II in which fungal protein kinases have been classified. mRNA levels for each isoform were measured during aerobic and anaerobic growth. The expression of each isoform is differential and associated to a particular growth stage. We reanalyzed the sequence of PKAC (GI 20218944), the only cloned sequence available until now for a catalytic subunit of M. circinelloides. PKAC cannot be classified as a PKA because of its difference in the conserved C-tail; it shares with PKB a conserved C2 domain in the N-terminus. No catalytic activity could be measured for this protein nor predicted bioinformatically. It can thus be classified as a pseudokinase. Its importance can not be underestimated since it is expressed at the mRNA level in different stages of growth, and its deletion is lethal. © 2016 British Mycological Society |
| author |
Ocampo, Josefina Rossi, Silvia Graciela Moreno, Silvia |
| author_facet |
Ocampo, Josefina Rossi, Silvia Graciela Moreno, Silvia |
| author_sort |
Ocampo, Josefina |
| title |
Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides |
| title_short |
Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides |
| title_full |
Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides |
| title_fullStr |
Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides |
| title_full_unstemmed |
Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides |
| title_sort |
multiple isoforms for the catalytic subunit of pka in the basal fungal lineage mucor circinelloides |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18786146_v120_n12_p1493_FernandezNunez http://hdl.handle.net/20.500.12110/paper_18786146_v120_n12_p1493_FernandezNunez |
| work_keys_str_mv |
AT ocampojosefina multipleisoformsforthecatalyticsubunitofpkainthebasalfungallineagemucorcircinelloides AT rossisilviagraciela multipleisoformsforthecatalyticsubunitofpkainthebasalfungallineagemucorcircinelloides AT morenosilvia multipleisoformsforthecatalyticsubunitofpkainthebasalfungallineagemucorcircinelloides |
| _version_ |
1768544294726008832 |