Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos
Probing dynamic processes occurring within the cell nucleus at the quantitative level has long been a challenge in mammalian biology. Advances in bio-imaging techniques over the past decade have enabled us to directly visualize nuclear processes in situ with unprecedented spatial and temporal resolu...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17417007_v14_n1_p_Zhao http://hdl.handle.net/20.500.12110/paper_17417007_v14_n1_p_Zhao |
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paper:paper_17417007_v14_n1_p_Zhao2023-06-08T16:27:00Z Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos Levi, Valeria animal cell nucleus embryology fluorescence microscopy gene expression regulation genetic epigenesis genetics mammal single cell analysis spatiotemporal analysis transcription initiation Animals Cell Nucleus Epigenesis, Genetic Gene Expression Regulation Mammals Microscopy, Fluorescence Single-Cell Analysis Spatio-Temporal Analysis Transcriptional Activation Probing dynamic processes occurring within the cell nucleus at the quantitative level has long been a challenge in mammalian biology. Advances in bio-imaging techniques over the past decade have enabled us to directly visualize nuclear processes in situ with unprecedented spatial and temporal resolution and single-molecule sensitivity. Here, using transcription as our primary focus, we survey recent imaging studies that specifically emphasize the quantitative understanding of nuclear dynamics in both time and space. These analyses not only inform on previously hidden physical parameters and mechanistic details, but also reveal a hierarchical organizational landscape for coordinating a wide range of transcriptional processes shared by mammalian systems of varying complexity, from single cells to whole embryos. © 2016 Plachta et al. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17417007_v14_n1_p_Zhao http://hdl.handle.net/20.500.12110/paper_17417007_v14_n1_p_Zhao |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
animal cell nucleus embryology fluorescence microscopy gene expression regulation genetic epigenesis genetics mammal single cell analysis spatiotemporal analysis transcription initiation Animals Cell Nucleus Epigenesis, Genetic Gene Expression Regulation Mammals Microscopy, Fluorescence Single-Cell Analysis Spatio-Temporal Analysis Transcriptional Activation |
spellingShingle |
animal cell nucleus embryology fluorescence microscopy gene expression regulation genetic epigenesis genetics mammal single cell analysis spatiotemporal analysis transcription initiation Animals Cell Nucleus Epigenesis, Genetic Gene Expression Regulation Mammals Microscopy, Fluorescence Single-Cell Analysis Spatio-Temporal Analysis Transcriptional Activation Levi, Valeria Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos |
topic_facet |
animal cell nucleus embryology fluorescence microscopy gene expression regulation genetic epigenesis genetics mammal single cell analysis spatiotemporal analysis transcription initiation Animals Cell Nucleus Epigenesis, Genetic Gene Expression Regulation Mammals Microscopy, Fluorescence Single-Cell Analysis Spatio-Temporal Analysis Transcriptional Activation |
description |
Probing dynamic processes occurring within the cell nucleus at the quantitative level has long been a challenge in mammalian biology. Advances in bio-imaging techniques over the past decade have enabled us to directly visualize nuclear processes in situ with unprecedented spatial and temporal resolution and single-molecule sensitivity. Here, using transcription as our primary focus, we survey recent imaging studies that specifically emphasize the quantitative understanding of nuclear dynamics in both time and space. These analyses not only inform on previously hidden physical parameters and mechanistic details, but also reveal a hierarchical organizational landscape for coordinating a wide range of transcriptional processes shared by mammalian systems of varying complexity, from single cells to whole embryos. © 2016 Plachta et al. |
author |
Levi, Valeria |
author_facet |
Levi, Valeria |
author_sort |
Levi, Valeria |
title |
Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos |
title_short |
Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos |
title_full |
Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos |
title_fullStr |
Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos |
title_full_unstemmed |
Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos |
title_sort |
quantitative imaging of mammalian transcriptional dynamics: from single cells to whole embryos |
publishDate |
2016 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17417007_v14_n1_p_Zhao http://hdl.handle.net/20.500.12110/paper_17417007_v14_n1_p_Zhao |
work_keys_str_mv |
AT levivaleria quantitativeimagingofmammaliantranscriptionaldynamicsfromsinglecellstowholeembryos |
_version_ |
1768546417766301696 |