GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes

Introduction: In the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal–epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et...

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Guardado en:
Detalles Bibliográficos
Publicado: 2015
Materias:
Adipocytes
Breast mammary gland
Cancer
Cell lines
Triglycerides
enzyme
galactose 3 O sulfotransferase 2
triacylglycerol
unclassified drug
CCAAT enhancer binding protein beta
Fabp4 protein, mouse
fatty acid binding protein
GAL3ST2 protein, human
Gal3St2 protein, mouse
sulfotransferase
sulfurtransferase
adipocyte
animal cell
Article
breast epithelium
cell differentiation
column chromatography
controlled study
genetic transfection
human
human cell
matrix assisted laser desorption ionization time of flight mass spectrometry
mouse
MTT assay
nonhuman
protein analysis
protein expression
two dimensional electrophoresis
Western blotting
3T3 cell line
adipogenesis
animal
cytology
female
HEK293 cell line
mass spectrometry
MCF 7 cell line
metabolism
tumor cell line
udder
3T3-L1 Cells
Adipogenesis
Animals
CCAAT-Enhancer-Binding Protein-beta
Cell Differentiation
Cell Line, Tumor
Fatty Acid-Binding Proteins
Female
HEK293 Cells
Humans
Mammary Glands, Animal
MCF-7 Cells
Mice
NIH 3T3 Cells
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sulfotransferases
Sulfurtransferases
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1699048X_v17_n7_p511_Guerra
http://hdl.handle.net/20.500.12110/paper_1699048X_v17_n7_p511_Guerra
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Introduction: In the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal–epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124–131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells. Materials and methods: Conditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line. Results: We described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells. Conclusions: Taken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity. © 2014, Federación de Sociedades Españolas de Oncología (FESEO).

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