Identification of novel transcriptional regulators of PKA subunits in Saccharomyces cerevisiae by quantitative promoter-reporter screening

The cAMP-dependent protein kinase (PKA) signaling is a broad pathway that plays important roles in the transduction of environmental signals triggering precise physiological responses. However, how PKA achieves the cAMP-signal transduction specificity is still in study. The regulation of expression...

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Autores principales:	Pautasso, Constanza, Reca, Sol Rita, Galello, Fiorella Ariadna, Portela, Paula, Zaremberg, Vanina, Rossi, Silvia Graciela
Publicado:	2016
Materias:	Bcy1 PKA Saccharomyces cerevisiae Tpks Transcription regulation choline cyclic AMP dependent protein kinase green fluorescent protein holoenzyme inositol phosphate Bcy1 protein, S cerevisiae cyclic AMP dependent protein kinase catalytic subunit Saccharomyces cerevisiae protein Article BCY1 gene enzyme active site enzyme phosphorylation enzyme subunit fungal gene gene ontology lipid metabolism nonhuman phosphate metabolism promoter region protein expression quantitative analysis signal transduction TPK1 gene TPK2 gene TPK3 gene transcription regulation enzymology gene expression profiling gene expression regulation gene fusion genetic transcription genetics metabolism reporter gene Artificial Gene Fusion Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Gene Expression Profiling Gene Expression Regulation, Fungal Genes, Reporter Promoter Regions, Genetic Saccharomyces cerevisiae Proteins Transcription, Genetic
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671356_v16_n5_p_Pautasso http://hdl.handle.net/20.500.12110/paper_15671356_v16_n5_p_Pautasso
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_15671356_v16_n5_p_Pautasso
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spelling	paper:paper_15671356_v16_n5_p_Pautasso2023-06-08T16:24:01Z Identification of novel transcriptional regulators of PKA subunits in Saccharomyces cerevisiae by quantitative promoter-reporter screening Pautasso, Constanza Reca, Sol Rita Galello, Fiorella Ariadna Portela, Paula Zaremberg, Vanina Rossi, Silvia Graciela Bcy1 PKA Saccharomyces cerevisiae Tpks Transcription regulation choline cyclic AMP dependent protein kinase green fluorescent protein holoenzyme inositol phosphate Bcy1 protein, S cerevisiae cyclic AMP dependent protein kinase catalytic subunit Saccharomyces cerevisiae protein Article BCY1 gene enzyme active site enzyme phosphorylation enzyme subunit fungal gene gene ontology lipid metabolism nonhuman phosphate metabolism promoter region protein expression quantitative analysis Saccharomyces cerevisiae signal transduction TPK1 gene TPK2 gene TPK3 gene transcription regulation enzymology gene expression profiling gene expression regulation gene fusion genetic transcription genetics metabolism reporter gene Saccharomyces cerevisiae Artificial Gene Fusion Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Gene Expression Profiling Gene Expression Regulation, Fungal Genes, Reporter Promoter Regions, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription, Genetic The cAMP-dependent protein kinase (PKA) signaling is a broad pathway that plays important roles in the transduction of environmental signals triggering precise physiological responses. However, how PKA achieves the cAMP-signal transduction specificity is still in study. The regulation of expression of subunits of PKA should contribute to the signal specificity. Saccharomyces cerevisiae PKA holoenzyme contains two catalytic subunits encoded by TPK1, TPK2 and TPK3 genes, and two regulatory subunits encoded by BCY1 gene. We studied the activity of these gene promoters using a fluorescent reporter synthetic genetic array screen, with the goal of systematically identifying novel regulators of expression of PKA subunits. Gene ontology analysis of the identified modulators showed enrichment not only in the category of transcriptional regulators, but also in less expected categories such as lipid and phosphate metabolism. Inositol, choline and phosphate were identified as novel upstream signals that regulate transcription of PKA subunit genes. The results support the role of transcription regulation of PKA subunits in cAMP specificity signaling. Interestingly, known targets of PKA phosphorylation are associated with the identified pathways opening the possibility of a reciprocal regulation. PKA would be coordinating different metabolic pathways and these processes would in turn regulate expression of the kinase subunits. © FEMS 2016. Fil:Pautasso, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Reca, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galello, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Portela, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zaremberg, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossi, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671356_v16_n5_p_Pautasso http://hdl.handle.net/20.500.12110/paper_15671356_v16_n5_p_Pautasso
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bcy1 PKA Saccharomyces cerevisiae Tpks Transcription regulation choline cyclic AMP dependent protein kinase green fluorescent protein holoenzyme inositol phosphate Bcy1 protein, S cerevisiae cyclic AMP dependent protein kinase catalytic subunit Saccharomyces cerevisiae protein Article BCY1 gene enzyme active site enzyme phosphorylation enzyme subunit fungal gene gene ontology lipid metabolism nonhuman phosphate metabolism promoter region protein expression quantitative analysis Saccharomyces cerevisiae signal transduction TPK1 gene TPK2 gene TPK3 gene transcription regulation enzymology gene expression profiling gene expression regulation gene fusion genetic transcription genetics metabolism reporter gene Saccharomyces cerevisiae Artificial Gene Fusion Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Gene Expression Profiling Gene Expression Regulation, Fungal Genes, Reporter Promoter Regions, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription, Genetic
spellingShingle	Bcy1 PKA Saccharomyces cerevisiae Tpks Transcription regulation choline cyclic AMP dependent protein kinase green fluorescent protein holoenzyme inositol phosphate Bcy1 protein, S cerevisiae cyclic AMP dependent protein kinase catalytic subunit Saccharomyces cerevisiae protein Article BCY1 gene enzyme active site enzyme phosphorylation enzyme subunit fungal gene gene ontology lipid metabolism nonhuman phosphate metabolism promoter region protein expression quantitative analysis Saccharomyces cerevisiae signal transduction TPK1 gene TPK2 gene TPK3 gene transcription regulation enzymology gene expression profiling gene expression regulation gene fusion genetic transcription genetics metabolism reporter gene Saccharomyces cerevisiae Artificial Gene Fusion Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Gene Expression Profiling Gene Expression Regulation, Fungal Genes, Reporter Promoter Regions, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription, Genetic Pautasso, Constanza Reca, Sol Rita Galello, Fiorella Ariadna Portela, Paula Zaremberg, Vanina Rossi, Silvia Graciela Identification of novel transcriptional regulators of PKA subunits in Saccharomyces cerevisiae by quantitative promoter-reporter screening
topic_facet	Bcy1 PKA Saccharomyces cerevisiae Tpks Transcription regulation choline cyclic AMP dependent protein kinase green fluorescent protein holoenzyme inositol phosphate Bcy1 protein, S cerevisiae cyclic AMP dependent protein kinase catalytic subunit Saccharomyces cerevisiae protein Article BCY1 gene enzyme active site enzyme phosphorylation enzyme subunit fungal gene gene ontology lipid metabolism nonhuman phosphate metabolism promoter region protein expression quantitative analysis Saccharomyces cerevisiae signal transduction TPK1 gene TPK2 gene TPK3 gene transcription regulation enzymology gene expression profiling gene expression regulation gene fusion genetic transcription genetics metabolism reporter gene Saccharomyces cerevisiae Artificial Gene Fusion Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Gene Expression Profiling Gene Expression Regulation, Fungal Genes, Reporter Promoter Regions, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription, Genetic
description	The cAMP-dependent protein kinase (PKA) signaling is a broad pathway that plays important roles in the transduction of environmental signals triggering precise physiological responses. However, how PKA achieves the cAMP-signal transduction specificity is still in study. The regulation of expression of subunits of PKA should contribute to the signal specificity. Saccharomyces cerevisiae PKA holoenzyme contains two catalytic subunits encoded by TPK1, TPK2 and TPK3 genes, and two regulatory subunits encoded by BCY1 gene. We studied the activity of these gene promoters using a fluorescent reporter synthetic genetic array screen, with the goal of systematically identifying novel regulators of expression of PKA subunits. Gene ontology analysis of the identified modulators showed enrichment not only in the category of transcriptional regulators, but also in less expected categories such as lipid and phosphate metabolism. Inositol, choline and phosphate were identified as novel upstream signals that regulate transcription of PKA subunit genes. The results support the role of transcription regulation of PKA subunits in cAMP specificity signaling. Interestingly, known targets of PKA phosphorylation are associated with the identified pathways opening the possibility of a reciprocal regulation. PKA would be coordinating different metabolic pathways and these processes would in turn regulate expression of the kinase subunits. © FEMS 2016.
author	Pautasso, Constanza Reca, Sol Rita Galello, Fiorella Ariadna Portela, Paula Zaremberg, Vanina Rossi, Silvia Graciela
author_facet	Pautasso, Constanza Reca, Sol Rita Galello, Fiorella Ariadna Portela, Paula Zaremberg, Vanina Rossi, Silvia Graciela
author_sort	Pautasso, Constanza
title	Identification of novel transcriptional regulators of PKA subunits in Saccharomyces cerevisiae by quantitative promoter-reporter screening
title_short	Identification of novel transcriptional regulators of PKA subunits in Saccharomyces cerevisiae by quantitative promoter-reporter screening
title_full	Identification of novel transcriptional regulators of PKA subunits in Saccharomyces cerevisiae by quantitative promoter-reporter screening
title_fullStr	Identification of novel transcriptional regulators of PKA subunits in Saccharomyces cerevisiae by quantitative promoter-reporter screening
title_full_unstemmed	Identification of novel transcriptional regulators of PKA subunits in Saccharomyces cerevisiae by quantitative promoter-reporter screening
title_sort	identification of novel transcriptional regulators of pka subunits in saccharomyces cerevisiae by quantitative promoter-reporter screening
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671356_v16_n5_p_Pautasso http://hdl.handle.net/20.500.12110/paper_15671356_v16_n5_p_Pautasso
work_keys_str_mv	AT pautassoconstanza identificationofnoveltranscriptionalregulatorsofpkasubunitsinsaccharomycescerevisiaebyquantitativepromoterreporterscreening AT recasolrita identificationofnoveltranscriptionalregulatorsofpkasubunitsinsaccharomycescerevisiaebyquantitativepromoterreporterscreening AT galellofiorellaariadna identificationofnoveltranscriptionalregulatorsofpkasubunitsinsaccharomycescerevisiaebyquantitativepromoterreporterscreening AT portelapaula identificationofnoveltranscriptionalregulatorsofpkasubunitsinsaccharomycescerevisiaebyquantitativepromoterreporterscreening AT zarembergvanina identificationofnoveltranscriptionalregulatorsofpkasubunitsinsaccharomycescerevisiaebyquantitativepromoterreporterscreening AT rossisilviagraciela identificationofnoveltranscriptionalregulatorsofpkasubunitsinsaccharomycescerevisiaebyquantitativepromoterreporterscreening
_version_	1768544198249676800

Identification of novel transcriptional regulators of PKA subunits in Saccharomyces cerevisiae by quantitative promoter-reporter screening

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