Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae)
Triatoma infestans, the main vector of Chagas disease in the southern cone countries, is the principal target of a regional elimination program. A better understanding of its dispersal, sources of reinfestation, and insecticide resistance is key to an effective control program. To address such probl...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v6_n1_p32_Marcet http://hdl.handle.net/20.500.12110/paper_15671348_v6_n1_p32_Marcet |
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paper:paper_15671348_v6_n1_p32_Marcet2023-06-08T16:24:01Z Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae) Chagas disease Microsatellites Reinfestation Triatoma infestans allele article Chagas disease controlled study disease carrier DNA library gene frequency gene locus gene sequence genetic variability microsatellite marker nonhuman nucleotide sequence parasite identification polymerase chain reaction priority journal Triatoma infestans Animals Chagas Disease Insect Vectors Microsatellite Repeats Triatoma Heteroptera Reduviidae Triatoma infestans Triatoma infestans, the main vector of Chagas disease in the southern cone countries, is the principal target of a regional elimination program. A better understanding of its dispersal, sources of reinfestation, and insecticide resistance is key to an effective control program. To address such problems, we identified and characterized 13 microsatellite loci of T. infestans. For each locus, primer sequences and PCR conditions are presented. Allele variability and frequency were analyzed in 59 T. infestans specimens from different rural communities in northwestern Argentina; nine loci were considered suitable for population genetic studies. Departure from Hardy-Weinberg equilibrium was detected in 10/13 loci with FIS values ranging from 0.04 to 0.91, indicating heterozygote deficit and a possible grade of sub-structure in the sample analyzed. Presence of null alleles in some loci cannot be discarded. The present work provides a promising tool to develop a population genetic study of natural populations of T. infestans in tandem with field studies and analyses of bug dispersal and the reinfestation process. © 2005 Elsevier B.V. All rights reserved. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v6_n1_p32_Marcet http://hdl.handle.net/20.500.12110/paper_15671348_v6_n1_p32_Marcet |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chagas disease Microsatellites Reinfestation Triatoma infestans allele article Chagas disease controlled study disease carrier DNA library gene frequency gene locus gene sequence genetic variability microsatellite marker nonhuman nucleotide sequence parasite identification polymerase chain reaction priority journal Triatoma infestans Animals Chagas Disease Insect Vectors Microsatellite Repeats Triatoma Heteroptera Reduviidae Triatoma infestans |
| spellingShingle |
Chagas disease Microsatellites Reinfestation Triatoma infestans allele article Chagas disease controlled study disease carrier DNA library gene frequency gene locus gene sequence genetic variability microsatellite marker nonhuman nucleotide sequence parasite identification polymerase chain reaction priority journal Triatoma infestans Animals Chagas Disease Insect Vectors Microsatellite Repeats Triatoma Heteroptera Reduviidae Triatoma infestans Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae) |
| topic_facet |
Chagas disease Microsatellites Reinfestation Triatoma infestans allele article Chagas disease controlled study disease carrier DNA library gene frequency gene locus gene sequence genetic variability microsatellite marker nonhuman nucleotide sequence parasite identification polymerase chain reaction priority journal Triatoma infestans Animals Chagas Disease Insect Vectors Microsatellite Repeats Triatoma Heteroptera Reduviidae Triatoma infestans |
| description |
Triatoma infestans, the main vector of Chagas disease in the southern cone countries, is the principal target of a regional elimination program. A better understanding of its dispersal, sources of reinfestation, and insecticide resistance is key to an effective control program. To address such problems, we identified and characterized 13 microsatellite loci of T. infestans. For each locus, primer sequences and PCR conditions are presented. Allele variability and frequency were analyzed in 59 T. infestans specimens from different rural communities in northwestern Argentina; nine loci were considered suitable for population genetic studies. Departure from Hardy-Weinberg equilibrium was detected in 10/13 loci with FIS values ranging from 0.04 to 0.91, indicating heterozygote deficit and a possible grade of sub-structure in the sample analyzed. Presence of null alleles in some loci cannot be discarded. The present work provides a promising tool to develop a population genetic study of natural populations of T. infestans in tandem with field studies and analyses of bug dispersal and the reinfestation process. © 2005 Elsevier B.V. All rights reserved. |
| title |
Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae) |
| title_short |
Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae) |
| title_full |
Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae) |
| title_fullStr |
Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae) |
| title_full_unstemmed |
Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae) |
| title_sort |
identification and characterization of microsatellite markers in the chagas disease vector triatoma infestans (heteroptera: reduviidae) |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_15671348_v6_n1_p32_Marcet http://hdl.handle.net/20.500.12110/paper_15671348_v6_n1_p32_Marcet |
| _version_ |
1768546320877879296 |