Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer

Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can al...

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Detalles Bibliográficos
Publicado: 1999
Materias:
alpha globin
DNA
fibronectin
messenger RNA
Pol protein
protein
RNA
transcription factor
article
binding site
cell line
enhancer region
exon
genetic linkage
human
molecular interaction
RNA splicing
transcription regulation
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10972765_v4_n2_p251_Cramer
http://hdl.handle.net/20.500.12110/paper_10972765_v4_n2_p251_Cramer
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Alternative mRNA splicing of the fibronectin EDI exon is controlled by a purine-rich exonic splicing enhancer (ESE), postulated as a binding site for SR proteins. By using a transient expression alternative splicing assay combined with promoter swapping, we have demonstrated that the promoter can also control EDI splicing, arguing for coupling between the transcription and splicing machineries. We now report that the SR proteins SF2/ASF and 9G8 stimulate EDI splicing in vivo and that their effect requires an intact EDI ESE. Most importantly, we show that sensitivity to these SR proteins critically depends on the promoter structure, suggesting that the transcription machinery modulates their recruitment to the ESE.

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