Possible role of carbonic anhydrase, V-H+-ATPase, and Cl -/HCO3 - exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus

We studied the participation of carbonic anhydrase (CA), V-H +-ATPase, and Cl-/HCO3 - exchanger in electrogenic ion absorption through the gills of Chasmagnathus granulatus. CA activity was measured in anterior gills and posterior gills after acclimation to 2‰, 10‰, 30‰ (about seawater), and 45‰ sal...

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Detalles Bibliográficos
Autores principales: Genovese, Griselda, Ortiz, Nicolás, Luquet, Carlos Marcelo
Publicado: 2005
Materias:
Carbonic anhydrase
Chasmagnathus granulatus
Cl-/HCO3 - exchanger
Crab
Electrogenic ion transport
Gills
H+-ATPase
Transepithelial potential difference
4 acetamido 4' isothiocyanatostilbene 2,2' disulfonic acid
acetazolamide
adenosine triphosphatase
bafilomycin A1
carbonate dehydratase
ouabain
absorption
acclimatization
animal tissue
article
controlled study
crab
cytosol
enzyme activity
gill
ion exchange
ion transport
microsome
nonhuman
osmosis
perfusion
salinity
Acetazolamide
Adenosine Triphosphatases
Animals
Biological Transport, Active
Brachyura
Carbonic Anhydrase Inhibitors
Carbonic Anhydrases
Chloride-Bicarbonate Antiporters
Electrophysiology
Ion Transport
Osmotic Pressure
Ouabain
Sodium Chloride
Animalia
Chasmagnathus granulata
Decapoda (Crustacea)
Strophanthus gratus
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10956433_v142_n3_p362_Genovese
http://hdl.handle.net/20.500.12110/paper_10956433_v142_n3_p362_Genovese
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:We studied the participation of carbonic anhydrase (CA), V-H +-ATPase, and Cl-/HCO3 - exchanger in electrogenic ion absorption through the gills of Chasmagnathus granulatus. CA activity was measured in anterior gills and posterior gills after acclimation to 2‰, 10‰, 30‰ (about seawater), and 45‰ salinity. The highest CA specific activity was detected in the microsomal fraction in anterior gills, and in the cytosolic fraction, in posterior ones. Both fractions were strongly induced by decreasing salinity only in posterior gills. Perfusion of posterior gills from crabs acclimated to either 2‰ or 10‰ with acetazolamide inhibited CA activity almost completely. In posterior gills from crabs acclimated to 2‰ and perfused with 20‰ saline (iso-osmotic for these crabs), acetazolamide reduced transepithelial potential difference (Vte) by 47%, further addition of ouabain enhanced the effect to 88%. Acetazolamide had no effect in the same gills perfused with 30‰ saline (iso-osmotic for seawater acclimated crabs). Bafilomycin A1 and SITS (inhibitors of V-H+-ATPase and Cl-/HCO3 -) reduced Vte by 15-16% in gills perfused with normal 20‰ saline, and by 77% and 45%, respectively when they were applied in Na-free 20‰ saline, suggesting the participation of those transporters and cytosolic CA in electrogenic ion absorption. © 2005 Elsevier Inc. All rights reserved.

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