Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria

Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and...

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Publicado: 2019
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DST
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1898_n_p27_Piuri
http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p27_Piuri
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spelling paper:paper_10643745_v1898_n_p27_Piuri2023-06-08T16:04:09Z Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria Antibiotics DST Fluoromycobacteriophage Mycobacteria Reporter phage Resistance carbenicillin cycloheximide ethambutol ethionamide isoniazid kanamycin rifampicin streptomycin tuberculostatic agent antibiotic sensitivity bacteriophage flow cytometry fluorescence microscopy fluoromycobacteriophage high throughput screening mycobacteriosis Mycobacterium Mycobacterium smegmatis infection nonhuman tuberculosis Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of Mycobacterium spp using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format. © 2019, Springer Science+Business Media, LLC, part of Springer Nature. 2019 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1898_n_p27_Piuri http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p27_Piuri
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antibiotics
DST
Fluoromycobacteriophage
Mycobacteria
Reporter phage
Resistance
carbenicillin
cycloheximide
ethambutol
ethionamide
isoniazid
kanamycin
rifampicin
streptomycin
tuberculostatic agent
antibiotic sensitivity
bacteriophage
flow cytometry
fluorescence microscopy
fluoromycobacteriophage
high throughput screening
mycobacteriosis
Mycobacterium
Mycobacterium smegmatis infection
nonhuman
tuberculosis
spellingShingle Antibiotics
DST
Fluoromycobacteriophage
Mycobacteria
Reporter phage
Resistance
carbenicillin
cycloheximide
ethambutol
ethionamide
isoniazid
kanamycin
rifampicin
streptomycin
tuberculostatic agent
antibiotic sensitivity
bacteriophage
flow cytometry
fluorescence microscopy
fluoromycobacteriophage
high throughput screening
mycobacteriosis
Mycobacterium
Mycobacterium smegmatis infection
nonhuman
tuberculosis
Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria
topic_facet Antibiotics
DST
Fluoromycobacteriophage
Mycobacteria
Reporter phage
Resistance
carbenicillin
cycloheximide
ethambutol
ethionamide
isoniazid
kanamycin
rifampicin
streptomycin
tuberculostatic agent
antibiotic sensitivity
bacteriophage
flow cytometry
fluorescence microscopy
fluoromycobacteriophage
high throughput screening
mycobacteriosis
Mycobacterium
Mycobacterium smegmatis infection
nonhuman
tuberculosis
description Fluoromycobacteriophages are a new class of reporter phages that contain Laboratorio fluorescent reporter genes (gfp, ZsYellow, and mCherry) and provide a simple means of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of Mycobacterium spp using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
title Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria
title_short Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria
title_full Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria
title_fullStr Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria
title_full_unstemmed Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria
title_sort fluoromycobacteriophages for drug susceptibility testing (dst) of mycobacteria
publishDate 2019
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1898_n_p27_Piuri
http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p27_Piuri
_version_ 1768546264053448704