Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach
A two-step process relying on cell encapsulation in alginate beads followed by inorganic gelation from colloidal metal oxides was successfully applied to the immobilization of Escherichia coli bacteria in the presence of boehmite and zirconium oxyhydroxide particles. In the case of the Al-based gel,...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09599428_v21_n22_p8026_Perullini http://hdl.handle.net/20.500.12110/paper_09599428_v21_n22_p8026_Perullini |
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paper:paper_09599428_v21_n22_p8026_Perullini2023-06-08T15:57:15Z Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach Perullini, Ana Mercedes Jobbagy, Matias Aldabe Bilmes, Sara Alfonsina Dora Alginate beads Cell encapsulations Colloidal metals Diffusion studies E. coli Escherichia coli bacteria Hybrid approach Hybrid gels Inorganic gelation Metal oxides Oxyhydroxides Positively charged Precursor solutions Two-step process Bacteria Bacteriology Biomolecules Cell immobilization Coagulation Escherichia coli Gelation Gels Metallic compounds Metals Oligomers Optimization Zirconium Alginate A two-step process relying on cell encapsulation in alginate beads followed by inorganic gelation from colloidal metal oxides was successfully applied to the immobilization of Escherichia coli bacteria in the presence of boehmite and zirconium oxyhydroxide particles. In the case of the Al-based gel, the alginate bead obtained at low biopolymer content provides an efficient barrier against the encapsulation stress. In contrast, an increase in the alginate concentration together with the phosphate-induced mineralization of the biopolymer bead is found necessary to maintain the viability of entrapped bacteria in Zr-based gels. Diffusion studies using model molecular and colloidal species put in evidence that positively charged Zr-oligomers and ZrO 2 nanoparticles may be involved in the cytotoxicity of the precursor solution. This optimization of the encapsulation process allows the first observation of E. coli growth within such metal oxide-alginate hybrid gels. Results presented in this work give a clear evidence that sol-gel based cell encapsulation can now be envisioned within a wide variety of metal oxide hosts through the optimization of the pre-encapsulation environment. © 2011 The Royal Society of Chemistry. Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jobbágy, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bilmes, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09599428_v21_n22_p8026_Perullini http://hdl.handle.net/20.500.12110/paper_09599428_v21_n22_p8026_Perullini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alginate beads Cell encapsulations Colloidal metals Diffusion studies E. coli Escherichia coli bacteria Hybrid approach Hybrid gels Inorganic gelation Metal oxides Oxyhydroxides Positively charged Precursor solutions Two-step process Bacteria Bacteriology Biomolecules Cell immobilization Coagulation Escherichia coli Gelation Gels Metallic compounds Metals Oligomers Optimization Zirconium Alginate |
| spellingShingle |
Alginate beads Cell encapsulations Colloidal metals Diffusion studies E. coli Escherichia coli bacteria Hybrid approach Hybrid gels Inorganic gelation Metal oxides Oxyhydroxides Positively charged Precursor solutions Two-step process Bacteria Bacteriology Biomolecules Cell immobilization Coagulation Escherichia coli Gelation Gels Metallic compounds Metals Oligomers Optimization Zirconium Alginate Perullini, Ana Mercedes Jobbagy, Matias Aldabe Bilmes, Sara Alfonsina Dora Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach |
| topic_facet |
Alginate beads Cell encapsulations Colloidal metals Diffusion studies E. coli Escherichia coli bacteria Hybrid approach Hybrid gels Inorganic gelation Metal oxides Oxyhydroxides Positively charged Precursor solutions Two-step process Bacteria Bacteriology Biomolecules Cell immobilization Coagulation Escherichia coli Gelation Gels Metallic compounds Metals Oligomers Optimization Zirconium Alginate |
| description |
A two-step process relying on cell encapsulation in alginate beads followed by inorganic gelation from colloidal metal oxides was successfully applied to the immobilization of Escherichia coli bacteria in the presence of boehmite and zirconium oxyhydroxide particles. In the case of the Al-based gel, the alginate bead obtained at low biopolymer content provides an efficient barrier against the encapsulation stress. In contrast, an increase in the alginate concentration together with the phosphate-induced mineralization of the biopolymer bead is found necessary to maintain the viability of entrapped bacteria in Zr-based gels. Diffusion studies using model molecular and colloidal species put in evidence that positively charged Zr-oligomers and ZrO 2 nanoparticles may be involved in the cytotoxicity of the precursor solution. This optimization of the encapsulation process allows the first observation of E. coli growth within such metal oxide-alginate hybrid gels. Results presented in this work give a clear evidence that sol-gel based cell encapsulation can now be envisioned within a wide variety of metal oxide hosts through the optimization of the pre-encapsulation environment. © 2011 The Royal Society of Chemistry. |
| author |
Perullini, Ana Mercedes Jobbagy, Matias Aldabe Bilmes, Sara Alfonsina Dora |
| author_facet |
Perullini, Ana Mercedes Jobbagy, Matias Aldabe Bilmes, Sara Alfonsina Dora |
| author_sort |
Perullini, Ana Mercedes |
| title |
Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach |
| title_short |
Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach |
| title_full |
Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach |
| title_fullStr |
Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach |
| title_full_unstemmed |
Improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach |
| title_sort |
improving bacteria viability in metal oxide hosts via an alginate-based hybrid approach |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09599428_v21_n22_p8026_Perullini http://hdl.handle.net/20.500.12110/paper_09599428_v21_n22_p8026_Perullini |
| work_keys_str_mv |
AT perullinianamercedes improvingbacteriaviabilityinmetaloxidehostsviaanalginatebasedhybridapproach AT jobbagymatias improvingbacteriaviabilityinmetaloxidehostsviaanalginatebasedhybridapproach AT aldabebilmessaraalfonsinadora improvingbacteriaviabilityinmetaloxidehostsviaanalginatebasedhybridapproach |
| _version_ |
1768544694244999168 |