The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro
In the present study, we obtained in vitro dual cultures between the liverwort Plagiochasma rupestre and two arbuscular mycorrhizal (AM) fungi: Glomus intraradices and Glomus clarum. Four agarized culture media were tested for optimal growth of P. rupestre. Also, a description of the symbiotic assoc...
Guardado en:
Autores principales: | , , , |
---|---|
Publicado: |
2012
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n12_p3393_Silvani http://hdl.handle.net/20.500.12110/paper_09593993_v28_n12_p3393_Silvani |
Aporte de: |
id |
paper:paper_09593993_v28_n12_p3393_Silvani |
---|---|
record_format |
dspace |
spelling |
paper:paper_09593993_v28_n12_p3393_Silvani2023-06-08T15:56:53Z The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro Silvani, Vanesa Analía Rodríguez, María Alejandra Godeas, Alicia Margarita Fracchia, Sebastian Arbuscular mycorrhiza Glomus Liverworts Monoxenic culture Plagiochasma rupestre Am fungus Arbuscular mycorrhizal Arbuscular mycorrhizal fungi Arbuscular mycorrhizas Culture media Fungal hyphae Fungal strains Germplasm collections Glomus Glomus intraradices Growth conditions In-vitro Liverworts Optimal growth Plagiochasma rupestre Symbiotic association Symbiotic partners Fungi Sugar (sucrose) Cell culture article chemistry culture medium cytology fungus spore Glomeromycota growth, development and aging liverwort microbiology mycelium mycorrhiza physiology symbiosis Culture Media Glomeromycota Hepatophyta Mycelium Mycorrhizae Spores, Fungal Symbiosis Arbuscular Bacteria (microorganisms) Fungi Glomus Glomus clarum Glomus intraradices Marchantiophyta Plagiochasma rupestre In the present study, we obtained in vitro dual cultures between the liverwort Plagiochasma rupestre and two arbuscular mycorrhizal (AM) fungi: Glomus intraradices and Glomus clarum. Four agarized culture media were tested for optimal growth of P. rupestre. Also, a description of the symbiotic association is provided. Plagiochasma rupestre gametophytes profusely grew axenically in MM with sucrose, and thalli were successfully subcultured under these growth conditions. Arbuscular mycorrhizal fungal hyphae colonized P. rupestre thalli through rhizoids or by forming appresoria in the ventral thallus cells. Arbuscules, mycelia and structures resembling intrathallic spores or vesicles were developed in the internal parenchymatic cells. The pattern of AM colonization in P. rupestre was very similar to the Paris-type. After 100 days of dual culture, the external mycelia of both AM fungal strains formed thousands of small viable spores, suggesting that P. rupestre in vitro culture could be a valuable tool for studying the biology of both symbiotic partners and conserving AM fungi in in vitro germplasm collections. © 2012 Springer Science+Business Media B.V. Fil:Silvani, V.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fracchia, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n12_p3393_Silvani http://hdl.handle.net/20.500.12110/paper_09593993_v28_n12_p3393_Silvani |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Arbuscular mycorrhiza Glomus Liverworts Monoxenic culture Plagiochasma rupestre Am fungus Arbuscular mycorrhizal Arbuscular mycorrhizal fungi Arbuscular mycorrhizas Culture media Fungal hyphae Fungal strains Germplasm collections Glomus Glomus intraradices Growth conditions In-vitro Liverworts Optimal growth Plagiochasma rupestre Symbiotic association Symbiotic partners Fungi Sugar (sucrose) Cell culture article chemistry culture medium cytology fungus spore Glomeromycota growth, development and aging liverwort microbiology mycelium mycorrhiza physiology symbiosis Culture Media Glomeromycota Hepatophyta Mycelium Mycorrhizae Spores, Fungal Symbiosis Arbuscular Bacteria (microorganisms) Fungi Glomus Glomus clarum Glomus intraradices Marchantiophyta Plagiochasma rupestre |
spellingShingle |
Arbuscular mycorrhiza Glomus Liverworts Monoxenic culture Plagiochasma rupestre Am fungus Arbuscular mycorrhizal Arbuscular mycorrhizal fungi Arbuscular mycorrhizas Culture media Fungal hyphae Fungal strains Germplasm collections Glomus Glomus intraradices Growth conditions In-vitro Liverworts Optimal growth Plagiochasma rupestre Symbiotic association Symbiotic partners Fungi Sugar (sucrose) Cell culture article chemistry culture medium cytology fungus spore Glomeromycota growth, development and aging liverwort microbiology mycelium mycorrhiza physiology symbiosis Culture Media Glomeromycota Hepatophyta Mycelium Mycorrhizae Spores, Fungal Symbiosis Arbuscular Bacteria (microorganisms) Fungi Glomus Glomus clarum Glomus intraradices Marchantiophyta Plagiochasma rupestre Silvani, Vanesa Analía Rodríguez, María Alejandra Godeas, Alicia Margarita Fracchia, Sebastian The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro |
topic_facet |
Arbuscular mycorrhiza Glomus Liverworts Monoxenic culture Plagiochasma rupestre Am fungus Arbuscular mycorrhizal Arbuscular mycorrhizal fungi Arbuscular mycorrhizas Culture media Fungal hyphae Fungal strains Germplasm collections Glomus Glomus intraradices Growth conditions In-vitro Liverworts Optimal growth Plagiochasma rupestre Symbiotic association Symbiotic partners Fungi Sugar (sucrose) Cell culture article chemistry culture medium cytology fungus spore Glomeromycota growth, development and aging liverwort microbiology mycelium mycorrhiza physiology symbiosis Culture Media Glomeromycota Hepatophyta Mycelium Mycorrhizae Spores, Fungal Symbiosis Arbuscular Bacteria (microorganisms) Fungi Glomus Glomus clarum Glomus intraradices Marchantiophyta Plagiochasma rupestre |
description |
In the present study, we obtained in vitro dual cultures between the liverwort Plagiochasma rupestre and two arbuscular mycorrhizal (AM) fungi: Glomus intraradices and Glomus clarum. Four agarized culture media were tested for optimal growth of P. rupestre. Also, a description of the symbiotic association is provided. Plagiochasma rupestre gametophytes profusely grew axenically in MM with sucrose, and thalli were successfully subcultured under these growth conditions. Arbuscular mycorrhizal fungal hyphae colonized P. rupestre thalli through rhizoids or by forming appresoria in the ventral thallus cells. Arbuscules, mycelia and structures resembling intrathallic spores or vesicles were developed in the internal parenchymatic cells. The pattern of AM colonization in P. rupestre was very similar to the Paris-type. After 100 days of dual culture, the external mycelia of both AM fungal strains formed thousands of small viable spores, suggesting that P. rupestre in vitro culture could be a valuable tool for studying the biology of both symbiotic partners and conserving AM fungi in in vitro germplasm collections. © 2012 Springer Science+Business Media B.V. |
author |
Silvani, Vanesa Analía Rodríguez, María Alejandra Godeas, Alicia Margarita Fracchia, Sebastian |
author_facet |
Silvani, Vanesa Analía Rodríguez, María Alejandra Godeas, Alicia Margarita Fracchia, Sebastian |
author_sort |
Silvani, Vanesa Analía |
title |
The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro |
title_short |
The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro |
title_full |
The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro |
title_fullStr |
The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro |
title_full_unstemmed |
The thalloid liverwort Plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro |
title_sort |
thalloid liverwort plagiochasma rupestre supports arbuscular mycorrhiza-like symbiosis in vitro |
publishDate |
2012 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n12_p3393_Silvani http://hdl.handle.net/20.500.12110/paper_09593993_v28_n12_p3393_Silvani |
work_keys_str_mv |
AT silvanivanesaanalia thethalloidliverwortplagiochasmarupestresupportsarbuscularmycorrhizalikesymbiosisinvitro AT rodriguezmariaalejandra thethalloidliverwortplagiochasmarupestresupportsarbuscularmycorrhizalikesymbiosisinvitro AT godeasaliciamargarita thethalloidliverwortplagiochasmarupestresupportsarbuscularmycorrhizalikesymbiosisinvitro AT fracchiasebastian thethalloidliverwortplagiochasmarupestresupportsarbuscularmycorrhizalikesymbiosisinvitro AT silvanivanesaanalia thalloidliverwortplagiochasmarupestresupportsarbuscularmycorrhizalikesymbiosisinvitro AT rodriguezmariaalejandra thalloidliverwortplagiochasmarupestresupportsarbuscularmycorrhizalikesymbiosisinvitro AT godeasaliciamargarita thalloidliverwortplagiochasmarupestresupportsarbuscularmycorrhizalikesymbiosisinvitro AT fracchiasebastian thalloidliverwortplagiochasmarupestresupportsarbuscularmycorrhizalikesymbiosisinvitro |
_version_ |
1768546593907146752 |