Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays
Cellular microarrays present a promising tool for multiplex evaluation of the signalling effect of substrate-immobilized factors on cellular differentiation. In this paper, we compare the early myoblast-to-osteoblast cell commitment steps in response to a growth factor stimulus using standard well p...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09574530_v24_n1_p189_RodriguezSegui http://hdl.handle.net/20.500.12110/paper_09574530_v24_n1_p189_RodriguezSegui |
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paper:paper_09574530_v24_n1_p189_RodriguezSegui2023-06-08T15:56:36Z Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays Cell differentiation Cellular differentiation Cellular microarrays Growth factor Microenvironments Osteoblast differentiation Well plates Cell proliferation Cell culture bone morphogenetic protein 2 fibronectin laminin transcription factor osterix article cell adhesion cell culture cell differentiation cell fate cell proliferation cell size controlled study human human cell immobilization immunohistochemistry microarray analysis microenvironment microtechnology molecular imaging myoblast osteoblast priority journal protein analysis protein expression signal transduction Animals Bone Morphogenetic Protein 2 Cell Differentiation Cellular Microenvironment CHO Cells Cricetinae Cricetulus Humans Mice Protein Array Analysis Cellular microarrays present a promising tool for multiplex evaluation of the signalling effect of substrate-immobilized factors on cellular differentiation. In this paper, we compare the early myoblast-to-osteoblast cell commitment steps in response to a growth factor stimulus using standard well plate differentiation assays or cellular microarrays. Our results show that restraints on the cell culture size, inherent to cellular microarrays, impair the differentiation outcome. Also, while cells growing on spots with immobilised BMP-2 are early biased towards the osteoblast fate, longer periods of cell culturing in the microarrays result in cell proliferation and blockage of osteoblast differentiation. The results presented here raise concerns about the efficiency of cell differentiation when the cell culture dimensions are reduced to a simplified microspot environment. Also, these results suggest that further efforts should be devoted to increasing the complexity of the microspots composition, aiming to replace signalling cues missing in this system. © 2012 Springer Science+Business Media New York. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09574530_v24_n1_p189_RodriguezSegui http://hdl.handle.net/20.500.12110/paper_09574530_v24_n1_p189_RodriguezSegui |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell differentiation Cellular differentiation Cellular microarrays Growth factor Microenvironments Osteoblast differentiation Well plates Cell proliferation Cell culture bone morphogenetic protein 2 fibronectin laminin transcription factor osterix article cell adhesion cell culture cell differentiation cell fate cell proliferation cell size controlled study human human cell immobilization immunohistochemistry microarray analysis microenvironment microtechnology molecular imaging myoblast osteoblast priority journal protein analysis protein expression signal transduction Animals Bone Morphogenetic Protein 2 Cell Differentiation Cellular Microenvironment CHO Cells Cricetinae Cricetulus Humans Mice Protein Array Analysis |
| spellingShingle |
Cell differentiation Cellular differentiation Cellular microarrays Growth factor Microenvironments Osteoblast differentiation Well plates Cell proliferation Cell culture bone morphogenetic protein 2 fibronectin laminin transcription factor osterix article cell adhesion cell culture cell differentiation cell fate cell proliferation cell size controlled study human human cell immobilization immunohistochemistry microarray analysis microenvironment microtechnology molecular imaging myoblast osteoblast priority journal protein analysis protein expression signal transduction Animals Bone Morphogenetic Protein 2 Cell Differentiation Cellular Microenvironment CHO Cells Cricetinae Cricetulus Humans Mice Protein Array Analysis Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays |
| topic_facet |
Cell differentiation Cellular differentiation Cellular microarrays Growth factor Microenvironments Osteoblast differentiation Well plates Cell proliferation Cell culture bone morphogenetic protein 2 fibronectin laminin transcription factor osterix article cell adhesion cell culture cell differentiation cell fate cell proliferation cell size controlled study human human cell immobilization immunohistochemistry microarray analysis microenvironment microtechnology molecular imaging myoblast osteoblast priority journal protein analysis protein expression signal transduction Animals Bone Morphogenetic Protein 2 Cell Differentiation Cellular Microenvironment CHO Cells Cricetinae Cricetulus Humans Mice Protein Array Analysis |
| description |
Cellular microarrays present a promising tool for multiplex evaluation of the signalling effect of substrate-immobilized factors on cellular differentiation. In this paper, we compare the early myoblast-to-osteoblast cell commitment steps in response to a growth factor stimulus using standard well plate differentiation assays or cellular microarrays. Our results show that restraints on the cell culture size, inherent to cellular microarrays, impair the differentiation outcome. Also, while cells growing on spots with immobilised BMP-2 are early biased towards the osteoblast fate, longer periods of cell culturing in the microarrays result in cell proliferation and blockage of osteoblast differentiation. The results presented here raise concerns about the efficiency of cell differentiation when the cell culture dimensions are reduced to a simplified microspot environment. Also, these results suggest that further efforts should be devoted to increasing the complexity of the microspots composition, aiming to replace signalling cues missing in this system. © 2012 Springer Science+Business Media New York. |
| title |
Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays |
| title_short |
Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays |
| title_full |
Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays |
| title_fullStr |
Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays |
| title_full_unstemmed |
Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays |
| title_sort |
simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09574530_v24_n1_p189_RodriguezSegui http://hdl.handle.net/20.500.12110/paper_09574530_v24_n1_p189_RodriguezSegui |
| _version_ |
1768543333419843584 |