Resistance Against Pepper Severe Mosaic Potyvirus in Transgenic Tobacco Plants

The coat protein gene of the pepper severe mosaic poty-virus was introduced into tobacco plants. Several transgenic lines were assayed for virus resistance under greenhouse conditions. Line 232, showing higher levels of resistance than other transgenic plants, was studied in further detail. Upon cha...

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Publicado: 1998
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09311785_v146_n7_p315_Rabinowicz
http://hdl.handle.net/20.500.12110/paper_09311785_v146_n7_p315_Rabinowicz
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09311785_v146_n7_p315_Rabinowicz
record_format dspace
spelling paper:paper_09311785_v146_n7_p315_Rabinowicz2025-07-30T18:29:04Z Resistance Against Pepper Severe Mosaic Potyvirus in Transgenic Tobacco Plants The coat protein gene of the pepper severe mosaic poty-virus was introduced into tobacco plants. Several transgenic lines were assayed for virus resistance under greenhouse conditions. Line 232, showing higher levels of resistance than other transgenic plants, was studied in further detail. Upon challenge with PepSMV, R 2 descendants from this line were monitored for virus accumulation for a period of 45 days. Virus titre was lower in plants of the line 232 than in control plants throughout the experiment. At 14 days post-inoculation the transgenic plant line reached the maximum virus accumulation measured by ELISA. Those plants also showed a 1 week delay of virus accumulation by comparison with control plants. After reaching the peak at 14 days post-inoculation the virus titre in line 232 decreased with respect to control plants, until the end of the experiment. These results are compatible with the presence of an RNA-mediated resistance mechanism. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09311785_v146_n7_p315_Rabinowicz http://hdl.handle.net/20.500.12110/paper_09311785_v146_n7_p315_Rabinowicz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description The coat protein gene of the pepper severe mosaic poty-virus was introduced into tobacco plants. Several transgenic lines were assayed for virus resistance under greenhouse conditions. Line 232, showing higher levels of resistance than other transgenic plants, was studied in further detail. Upon challenge with PepSMV, R 2 descendants from this line were monitored for virus accumulation for a period of 45 days. Virus titre was lower in plants of the line 232 than in control plants throughout the experiment. At 14 days post-inoculation the transgenic plant line reached the maximum virus accumulation measured by ELISA. Those plants also showed a 1 week delay of virus accumulation by comparison with control plants. After reaching the peak at 14 days post-inoculation the virus titre in line 232 decreased with respect to control plants, until the end of the experiment. These results are compatible with the presence of an RNA-mediated resistance mechanism.
title Resistance Against Pepper Severe Mosaic Potyvirus in Transgenic Tobacco Plants
spellingShingle Resistance Against Pepper Severe Mosaic Potyvirus in Transgenic Tobacco Plants
title_short Resistance Against Pepper Severe Mosaic Potyvirus in Transgenic Tobacco Plants
title_full Resistance Against Pepper Severe Mosaic Potyvirus in Transgenic Tobacco Plants
title_fullStr Resistance Against Pepper Severe Mosaic Potyvirus in Transgenic Tobacco Plants
title_full_unstemmed Resistance Against Pepper Severe Mosaic Potyvirus in Transgenic Tobacco Plants
title_sort resistance against pepper severe mosaic potyvirus in transgenic tobacco plants
publishDate 1998
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09311785_v146_n7_p315_Rabinowicz
http://hdl.handle.net/20.500.12110/paper_09311785_v146_n7_p315_Rabinowicz
_version_ 1840321046313435136

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