Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions
The effects of different experimental conditions on the antiviral activity of the sulphated xylomannan F6, isolated from the red seaweed Nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) were studied. The compound was equally effective against seve...
| Autores principales: | , , |
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| Publicado: |
1995
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n2_p91_Pujol http://hdl.handle.net/20.500.12110/paper_03257541_v27_n2_p91_Pujol |
| Aporte de: |
| Sumario: | The effects of different experimental conditions on the antiviral activity of the sulphated xylomannan F6, isolated from the red seaweed Nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) were studied. The compound was equally effective against several strains of HSV-1 (F, KOS, B-2006 and Field) and HSV-2 (G) in different cell lines (Vero, HEp-2 and BHK-21). Furthermore, the antiviral activity of F6 was independent of the method employed to determine the 50% inhibitory concentration (IC50) (virus yield or plaque reduction tests). The value of the IC50 against HSV-1 was not significantly affected by the initial virus concentration since a variation in the multiplicity of infection from 0.0001 to 0.1 UFP/cell only increased twofold the IC50 from 0.61 to 1.35 micrograms/ml, respectively. The potent inhibitory effect of F6 against high doses of HSV-1 represents a significant advantage for this sulphated polysaccharide with respect to other compounds only active against low viral inocula. |
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