Innate immune response between environmental factors and abortion: Lipopolysaccharide tolerization model during gestation
The exacerbated local inflammatory response that triggers immunological abortion on CBA/j x DBA/2 murine model depends on environmental factors such as LPS. Since deficient Tlr4 mice are resistant to LPS-induced abortion, the effect of LPS tolerization on the prevention of fetal losses, as well as o...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v40_n1_p71_Dubinsky http://hdl.handle.net/20.500.12110/paper_03252957_v40_n1_p71_Dubinsky |
| Aporte de: |
| Sumario: | The exacerbated local inflammatory response that triggers immunological abortion on CBA/j x DBA/2 murine model depends on environmental factors such as LPS. Since deficient Tlr4 mice are resistant to LPS-induced abortion, the effect of LPS tolerization on the prevention of fetal losses, as well as on the synthesis of placental cytokines on CBA/j x DBA/2 model has been studied. Low doses of LPS were inoculated during pre and post-implantatory stages on CBA/j pregnant mice. In order to investigate tolerization effect, abortogenic doses of LPS were added to placental and splenocyte cul-Results obtained with post-implantatory inoculation of 1μg/day LPS showed a decrease in the abortion rate as well as an IL-6 hyporesponsive to LPS booster, both in placenta and splenocyte cultures. However, stimulation with abortogenic dose of LPS modified TNF-α levels by an increase in placenta but a decrease in splenocyte culture supernatants. Considering these results, it can be concluded that LPS tolerance induction depends on the organ as well as on the cytokine studied. |
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