Biochemical and biophysical studies of Bacillus subtilis envelopes under hyperosmotic stress

The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a h...

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Detalles Bibliográficos
Publicado: 2000
Materias:
Bacillus subtilis
Fatty acids
Membranes
Phospholipids
Stress
fatty acid
membrane lipid
phosphatidylglycerol
phospholipid
adaptation
bacterial membrane
conference paper
fluorescence
hydrophobicity
lipid analysis
lipid composition
membrane fluidity
nonhuman
osmotic stress
Cell Membrane Permeability
Fatty Acids
Membrane Fluidity
Membrane Lipids
Water-Electrolyte Balance
Luria
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v55_n1-3_p137_Lopez
http://hdl.handle.net/20.500.12110/paper_01681605_v55_n1-3_p137_Lopez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The behaviour and state of the envelopes from B. subtilis cultures grown in Luria Bertani (LB) medium with and without 1.5 M NaCl are compared. Under hypertonic conditions, the hydrophobicity of the cultures increases. The phospholipid and fatty acid (FA) compositions show important differences: a higher cardiolipin (CL) content [at the expense of phosphatidylglycerol (PG)], and a higher unsaturated and straight chain FA content. The fluidity of the membranes, determined with fluorescent probes, indicates an increase in viscosity of the cytoplasmic membrane. The consequences of these variations in membrane permeability and osmotolerance are discussed. Copyright (C) 2000 Elsevier Science B.V.

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