Phosphoenolpyruvate carboxykinase from Trypanosoma cruzi. Purification and physicochemical and kinetic properties

Phosphoenolpyruvate carboxykinase (PEPCK) has been purified to homogeneity from epimastigotes of the Tul 0 strain of Trypanosoma cruzi. The physicochemical parameters determined allowed the calculation of an average molecular mass of 120 kDa; the subunit molecular mass, about 61 kDa, is in good agre...

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Detalles Bibliográficos
Autores principales: Cymeryng, Cora Beatriz, Cannata, Joaquín J.B.
Publicado: 1995
Materias:
Aerobic fermentation
Glucose
Phosphoenolpyruvate carboxykinase
Trypanosoma cruzi
glucose
phosphoenolpyruvate carboxykinase (gtp)
animal experiment
article
carboxylation
controlled study
enzyme analysis
enzyme purification
fermentation
nonhuman
physical chemistry
priority journal
trypanosoma cruzi
Animal
Cations, Divalent
Chemistry, Physical
Enzyme Inhibitors
Hydrogen-Ion Concentration
Kinetics
Molecular Weight
NAD
Nucleotides
Phosphoenolpyruvate Carboxykinase (GTP)
Protein Conformation
Substrate Specificity
Support, Non-U.S. Gov't
Animalia
Trypanosoma
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v73_n1-2_p91_Cymeryng
http://hdl.handle.net/20.500.12110/paper_01666851_v73_n1-2_p91_Cymeryng
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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