Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8

Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene...

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Detalles Bibliográficos
Autores principales: Pettinari, María Julia, Vazquez, Gustavo, Silberschmidt, Daniel, Méndez, Beatriz Silvia
Publicado: 2001
Materias:
acetoacetyl coenzyme a reductase
acetoacetyl-CoA reductase
acetyl coenzyme A acyltransferase
acyltransferase
alcohol dehydrogenase
hydroxybutyric acid
poly beta hydroxybutyrate polymerase
poly(3 hydroxybutyric acid)
poly-beta-hydroxybutyrate
poly-beta-hydroxybutyrate polymerase
polyester
article
Azotobacter
bacterial gene
DNA sequence
gene deletion
genetics
growth, development and aging
metabolism
molecular cloning
molecular genetics
Acetyl-CoA C-Acyltransferase
Acyltransferases
Alcohol Oxidoreductases
Cloning, Molecular
Gene Deletion
Genes, Bacterial
Hydroxybutyrates
Molecular Sequence Data
Polyesters
Sequence Analysis, DNA
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v67_n3-12_p5331_Pettinari
http://hdl.handle.net/20.500.12110/paper_00992240_v67_n3-12_p5331_Pettinari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Raktonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or oclanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chuin-length hydroxyalkanoates into PHB.

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