Release and specific binding of prostaglandins in bovine pineal gland
Incubated bovine pineal glands released prostaglandin E- and prostaglandin F-like material (304 ± 20 and 582 ± 56 pg/mg dry tissue wt/h, respectively) and the release was increased 2.2 2.9-fold by adding 10-4-10-6M of norepinephrine to the medium. Binding assays revealed the existence of high affini...
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1979
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00906980_v18_n4_p577_Cardinali http://hdl.handle.net/20.500.12110/paper_00906980_v18_n4_p577_Cardinali |
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paper:paper_00906980_v18_n4_p577_Cardinali |
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paper:paper_00906980_v18_n4_p577_Cardinali2023-06-08T15:07:52Z Release and specific binding of prostaglandins in bovine pineal gland Norepinephrine Pineal Gland Prostaglandin High Affinity Binding Prostaglandin Receptors Sympathetic Nervous System indometacin noradrenalin prostaglandin prostaglandin e1 prostaglandin e2 radioisotope animal experiment cattle drug binding drug release endocrine system in vitro study pineal body prostaglandin e2 h 3 prostaglandin f2 alpha h 3 radioimmunoassay Animal Cattle Culture Media Norepinephrine Pineal Gland Prostaglandins E Prostaglandins F Receptors, Prostaglandin Secretory Rate Incubated bovine pineal glands released prostaglandin E- and prostaglandin F-like material (304 ± 20 and 582 ± 56 pg/mg dry tissue wt/h, respectively) and the release was increased 2.2 2.9-fold by adding 10-4-10-6M of norepinephrine to the medium. Binding assays revealed the existence of high affinity binding of 3H-prostaglandin E2 (3H-PGE2) and 3H-prostaglandin F2α (3H-PGF2α) in low speed supernatants of pineal homogenates. Binding was increased by increasing Ca++ concentration in medium up to 2 mM, was heat-labile and was depressed following incubation with trypsin. In subcellular fractionation studies maximal 3H-PG binding was found in the 27000 × g pellet. Scatchard analysis of 3H-PGE2 binding revealed the presence of a single population of binding sites with a Kd= 1.2 nM and a binding site concentration of 1-2 pmoles/g protein. A single population of binding sites for 3H-PGF2α was also detected with a Kd= 1.7 nM and a similar binding site concentration. Non-radioactive PGE1 and PGE2 were almost equally effective to compete for 3H-PGE2 binding sites (ED50= 5 and 2 nM, respectively). Unlabeled PGF2 was relatively ineffective to compete for 3H-PGE2 binding (ED50>1000 nM) but displaced effectively 3H-PGF2α binding (ED50= 1.2 nM). © 1979. 1979 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00906980_v18_n4_p577_Cardinali http://hdl.handle.net/20.500.12110/paper_00906980_v18_n4_p577_Cardinali |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Norepinephrine Pineal Gland Prostaglandin High Affinity Binding Prostaglandin Receptors Sympathetic Nervous System indometacin noradrenalin prostaglandin prostaglandin e1 prostaglandin e2 radioisotope animal experiment cattle drug binding drug release endocrine system in vitro study pineal body prostaglandin e2 h 3 prostaglandin f2 alpha h 3 radioimmunoassay Animal Cattle Culture Media Norepinephrine Pineal Gland Prostaglandins E Prostaglandins F Receptors, Prostaglandin Secretory Rate |
| spellingShingle |
Norepinephrine Pineal Gland Prostaglandin High Affinity Binding Prostaglandin Receptors Sympathetic Nervous System indometacin noradrenalin prostaglandin prostaglandin e1 prostaglandin e2 radioisotope animal experiment cattle drug binding drug release endocrine system in vitro study pineal body prostaglandin e2 h 3 prostaglandin f2 alpha h 3 radioimmunoassay Animal Cattle Culture Media Norepinephrine Pineal Gland Prostaglandins E Prostaglandins F Receptors, Prostaglandin Secretory Rate Release and specific binding of prostaglandins in bovine pineal gland |
| topic_facet |
Norepinephrine Pineal Gland Prostaglandin High Affinity Binding Prostaglandin Receptors Sympathetic Nervous System indometacin noradrenalin prostaglandin prostaglandin e1 prostaglandin e2 radioisotope animal experiment cattle drug binding drug release endocrine system in vitro study pineal body prostaglandin e2 h 3 prostaglandin f2 alpha h 3 radioimmunoassay Animal Cattle Culture Media Norepinephrine Pineal Gland Prostaglandins E Prostaglandins F Receptors, Prostaglandin Secretory Rate |
| description |
Incubated bovine pineal glands released prostaglandin E- and prostaglandin F-like material (304 ± 20 and 582 ± 56 pg/mg dry tissue wt/h, respectively) and the release was increased 2.2 2.9-fold by adding 10-4-10-6M of norepinephrine to the medium. Binding assays revealed the existence of high affinity binding of 3H-prostaglandin E2 (3H-PGE2) and 3H-prostaglandin F2α (3H-PGF2α) in low speed supernatants of pineal homogenates. Binding was increased by increasing Ca++ concentration in medium up to 2 mM, was heat-labile and was depressed following incubation with trypsin. In subcellular fractionation studies maximal 3H-PG binding was found in the 27000 × g pellet. Scatchard analysis of 3H-PGE2 binding revealed the presence of a single population of binding sites with a Kd= 1.2 nM and a binding site concentration of 1-2 pmoles/g protein. A single population of binding sites for 3H-PGF2α was also detected with a Kd= 1.7 nM and a similar binding site concentration. Non-radioactive PGE1 and PGE2 were almost equally effective to compete for 3H-PGE2 binding sites (ED50= 5 and 2 nM, respectively). Unlabeled PGF2 was relatively ineffective to compete for 3H-PGE2 binding (ED50>1000 nM) but displaced effectively 3H-PGF2α binding (ED50= 1.2 nM). © 1979. |
| title |
Release and specific binding of prostaglandins in bovine pineal gland |
| title_short |
Release and specific binding of prostaglandins in bovine pineal gland |
| title_full |
Release and specific binding of prostaglandins in bovine pineal gland |
| title_fullStr |
Release and specific binding of prostaglandins in bovine pineal gland |
| title_full_unstemmed |
Release and specific binding of prostaglandins in bovine pineal gland |
| title_sort |
release and specific binding of prostaglandins in bovine pineal gland |
| publishDate |
1979 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00906980_v18_n4_p577_Cardinali http://hdl.handle.net/20.500.12110/paper_00906980_v18_n4_p577_Cardinali |
| _version_ |
1768543270557712384 |